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Volume 18, Issue 4
  • ISSN: 2667-3878
  • E-ISSN: 2667-3886

Abstract

Background

Autophagy plays a crucial role in modulating the proliferation of cancer diseases. However, the application of Naringenin (Nar), a compound with potential benefits against these diseases, has been limited due to its poor solubility and bioavailability.

Objective

This study aimed to develop solid lipid nanoparticles (Nar-SLNs) loaded with Nar to enhance their therapeutic impact.

Methods

experiments using Rin-5F cells exposed to Nar and Nar-SLNs were carried out to investigate the protective effects of Nar and its nanoformulation against the pancreatic cancer cell line of Rin-5F.

Results

Treatment with Nar and Nar-SLN led to an increase in autophagic markers (Akt, LC3, Beclin1, and ATG genes) and a decrease in the level of miR-21. Both Nar and Nar-SLN treatments inhibited cell proliferation and reduced the expression of autophagic markers. Notably, Nar-SLNs exhibited greater efficacy compared to free Nar.

Conclusion

These findings suggest that SLNs effectively enhance the cytotoxic impact of Nar, making Nar-SLNs a promising candidate for suppressing or preventing Rin-5F cell growth.

© 2024 Bentham Science Publishers
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2024-12-01
2024-11-15

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/content/journals/raddf/10.2174/0126673878297658240804192222
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Cytotoxic Impact of Naringenin-Loaded Solid Lipid Nanoparticles on RIN5F Pancreatic β Cells via Autophagy Blockage
Recent Advances in Drug Delivery and Formulation 18, 304 (2024); https://doi.org/10.2174/0126673878297658240804192222
/content/journals/raddf/10.2174/0126673878297658240804192222
/content/journals/raddf/10.2174/0126673878297658240804192222
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  • Article Type:     Research Article
Keyword(s): Autophagy; DNA mutations; miRNA; naringenin-loaded solid lipid nanoparticle; pancreatic cancer; Rin-5F cells

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