The Potency of Wound Healing of Nanogel-containing Mikania micrantha Leaves Extract in Hyperglycemic Rats

Introduction: Mikania micrantha has been traditionally used for wound dressings and to promote the healing of sores. This is due to the content of alkaloids and terpenoids/steroids compounds. Hyperglycemia is a good medium for bacterial growth that inhibits the wound healing process. Purpose: This study aimed to determine the wound healing of nanogels containing MMLE in hyperglycemic rats as a model for diabetic wounds. Methods: Mikania micrantha leaves were extracted with the maceration method using 96% ethanol in 5 days. Carbopol 940 was used as the gelling agent. The parameters observed during the physical testing of nanogels were organoleptic, homogeneity, pH, and size of the particle. Antibacterial activity was tested on Staphylococcus aureus, Staphylococcus epidermis, and Escherichia coli. Moreover, wound healing activity was tested in hyperglycemic rats after observing for 14 days. Diabetic wound healing was treated with 4 groups (P1, P2, K1, K2). Data were analyzed using SPSS. Results: Nanogel showed homogeneity, dark green color, transparency, pH 6.1± 0.1, and particle size range in 255-456 nm. The inhibition zones of antibacterial testing, i.e., Staphylococcus aureus, Staphylococcus epidermis, and Escherichia coli, were 10.57 ± 0.26 mm, 9.73 ± 0.21 mm, and 8.4 ± 0.1 mm. The percentage of diabetic wound healing was in the range of 92.79±3.81% to 94.08 ± 2.33% for 14 days of observation. Conclusion: MMLE nanogels have the potential as a treatment for diabetic wound healing.