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Volume 13, Issue 1
  • ISSN: 2211-7385
  • E-ISSN: 2211-7393

Abstract

Objectives

Resveratrol (Res) is a bifunctional compound found in numerous plants, including grapes and mulberries. Nanotechnology has promising applications in medicine. The ability of various nanomaterials to serve as radiosensitizers against tumor cells were reported in several manuscripts. The present investigation aimed to assess the antitumor and radiosensitizing effects of Res-CMCNPs on EAC-bearing mice.

Methods

Res-CMCNPs have been developed using the CMC emulsification cross-linking technique. Entrapment efficiency (%), particle size, Polydispersity index and ZETA potential, UV, FT-IR spectra, and drug release were evaluated and described for RES-CMCNPs. The radiosensitizing properties of RES-CMCNPs were also evaluated and against EAC-carrying rodents. The LD of Res-CMCNPs was estimated and its 1/20 LD50 was prepared for treating EAC transplanted mice.

Results

The results revealed that the Res-CMCNPs exhibited a high entrapment efficiency (85.46%) and a size of approximately 184.60 ±17.36 nm with zeta potential value equals -51.866 mv. Also, the UV spectra of Res and Res-CMCNPs have strong absorption at 225 and 290 nm. The percentage of resveratrol release at pHs 5.8 and 7.4 was found to be 56.73% and 51.60%, respectively, after 24 h at 100 rpm. Also, the FTIR analysis confirmed the chemical stability of resveratrol in Res-CMCNPs cross-linking. The IC values of Res-CMCNPs against EAC cells viability were 32.99, 25.46, and 22.21 µg after 24-, 48- and 72 h incubation, respectively, whereas those of Res-CMCNPs in combination with γ-irradiation after 6-, 10 and 12-mins exposure were 24.07, 16.06 and 7.48 µg, respectively. Also, the LD50 of Res-CMCNPs was 2180 mg/kg.b.w. The treatment of EAC-bearing mice with Res-CMCNPs plus γ-irradiation improved plasma levels of NO, caspase-3, P53 and NF-kB levels as well as liver MDA, GSH, SOD, CAT, LT-B4, aromatase, Bax, Bcl2 and TGF-β levels and exhibited more significant anticancer activity than administration of Res-CMCNPs and/or exposure to γ-irradiation individually. On the other hand, administration of Res-CMCNPs in combination with γ-irradiation attenuated liver mRNAs (21, 29b, 181a, and 451) gene expression.

Conclusion

Grafting resveratrol onto carboxymethyl chitosan appears to be a promising strategy for cancer therapy as a radiosensitizer, potentiating tumor cells' sensitivity to radiation by improving levels of proinflammatory features and antioxidant biomarkers.

© 2025 Bentham Science Publishers
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2025-01-10

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References

  1. Cancer.Available from: https://www.who.int/news-room/fact-sheets/detail/cancer (accessed on 6 June 2023).
  2. Rodriguez-CasadoA. The health potential of fruits and vegetables phytochemicals: Notable examples.Crit. Rev. Food Sci. Nutr.20165671097110710.1080/10408398.2012.75514925225771
     [Google Scholar]
  3. NoratT. ScocciantiC. Boutron-RuaultM.C. European code against cancer. In: Diet and Cancer. 4th ed. Cancer Epidemiol.201539S5666
     [Google Scholar]
  4. WilliamsM.T. HordN.G. The role of dietary factors in cancer prevention: Beyond fruits and vegetables.Nutr. Clin. Pract.200520445145910.1177/011542650502000445116207684
     [Google Scholar]
  5. RudzińskaA. JuchaniukP. OberdaJ. Phytochemicals in cancer treatment and cancer prevention—review on epidemiological data and clinical trials.Nutrients2023158189610.3390/nu1508189637111115
     [Google Scholar]
  6. CaraK.C. GoldmanD.M. KollmanB.K. AmatoS.S. TullM.D. KarlsenM.C. Commonalities among dietary recommendations from 2010 to 2021 clinical practice guidelines: A meta-epidemiological study from the american college of lifestyle medicine.Adv. Nutr.202314350051510.1016/j.advnut.2023.03.00736940903
     [Google Scholar]
  7. VieiraI.R.S. Conte-JuniorC.A. Nano-delivery systems for food bioactive compounds in cancer: Prevention, therapy, and clinical applications.Crit. Rev. Food Sci. Nutr.20226126
     [Google Scholar]
  8. LucciP. SaurinaJ. Núñٌْez O. Trends in LC-MS and LC-HRMS analysis and characterization of polyphenols in food.Trends Analyt. Chem.20178812410.1016/j.trac.2016.12.006
     [Google Scholar]
  9. VestergaardM. IngmerH. Antibacterial and antifungal properties of resveratrol.Int. J. Antimicrob. Agents201953671672310.1016/j.ijantimicag.2019.02.01530825504
     [Google Scholar]
  10. CioneE. La TorreC. CannataroR. CaroleoM.C. PlastinaP. GallelliL. Quercetin, epigallocatechin gallate, curcumin, and resveratrol: From dietary sources to human MicroRNA modulation.Molecules20192516310.3390/molecules2501006331878082
     [Google Scholar]
  11. LiangC. YiK. ZhouX. Destruction of the cellular antioxidant pool contributes to resveratrol‐induced senescence and apoptosis in lung cancer.Phytother. Res.20233772995300810.1002/ptr.779536866538
     [Google Scholar]
  12. WangP. WuQ. PengQ. Comparative analysis of the molecular mechanism of inhibiting proliferation and migration in cervical cancer HeLa cell by curcumin and resveratrol.Nat. Prod. Res.2022231636597703
     [Google Scholar]
  13. KabirS.R. DaiZ. NurujjamanM. Biogenic silver/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo.J. Cell. Mol. Med.20202422132231323410.1111/jcmm.1593433047886
     [Google Scholar]
  14. AygünA. GülbağçaF. NasM.S. Biological synthesis of silver nanoparticles using Rheum ribes and evaluation of their anticarcinogenic and antimicrobial potential: A novel approach in phytonanotechnology.J. Pharm. Biomed. Anal.202017911301210.1016/j.jpba.2019.11301231791838
     [Google Scholar]
  15. AygünA. ÖzdemirS. GülcanM. CellatK. ŞenF. Synthesis and characterization of Reishi mushroom-mediated green synthesis of silver nanoparticles for the biochemical applications.J. Pharm. Biomed. Anal.202017811297010.1016/j.jpba.2019.11297031722822
     [Google Scholar]
  16. KabirS.R. IslamF. AsaduzzamanA.K.M. Biogenic silver/silver chloride nanoparticles inhibit human cancer cells proliferation in vitro and Ehrlich ascites carcinoma cells growth in vivo.Sci. Rep.2022121890910.1038/s41598‑022‑12974‑z35618812
     [Google Scholar]
  17. ZhuX. YuZ. FengL. DengL. FangZ. LiuZ. Chitosan-based nanoparticle co-delivery of docetaxel and curcumin ameliorates anti-tumor chemoimmunotherapy in lung cancer.Carbohydr. Polym.20212686511823710.1016/j.carbpol.2021.118237
     [Google Scholar]
  18. GrossoR. de-PazM.V. Thiolated-polymer-based nanoparticles as an avant-garde approach for anticancer therapies—reviewing thiomers from chitosan and hyaluronic acid.Pharmaceutics202113685410.3390/pharmaceutics1306085434201403
     [Google Scholar]
  19. HerdianaY. WathoniN. ShamsuddinS. JoniI.M. MuchtaridiM. Chitosan-based nanoparticles of targeted drug delivery system in breast cancer treatment.Polymers20211311171710.3390/polym1311171734074020
     [Google Scholar]
  20. ZengZ. LiuY. WenQ. Experimental study on preparation and anti-tumor efficiency of nanoparticles targeting M2 macrophages.Drug Deliv.202128194395610.1080/10717544.2021.192107633988472
     [Google Scholar]
  21. MostafaM.M. AminM.M. ZakariaM.Y. HusseinM.A. ShamaaM.M. Abd El-HalimS.M. Chitosan surface-modified PLGA nanoparticles loaded with cranberry powder extract as a potential oral delivery platform for targeting colon cancer cells.Pharmaceutics202315260610.3390/pharmaceutics1502060636839928
     [Google Scholar]
  22. MohamadE.A. MohamedZ.N. HusseinM.A. ElneklawiM.S. GANE can improve lung fibrosis by reducing inflammation via Promoting p38MAPK/TGF-β1/NF-κB signaling pathway downregulation.ACS Omega2022733109312010.1021/acsomega.1c0659135097306
     [Google Scholar]
  23. M SolimanS MosallamS MamdouhMA HusseinMA M Abd El-HalimS. Design and optimization of cranberry extract loaded bile salt augmented liposomes for targeting of MCP-1/STAT3/VEGF signaling pathway in DMN-intoxicated liver in rats.Drug Deliv.202229142743910.1080/10717544.2022.203287535098843
     [Google Scholar]
  24. ElneklawiM.S. MohamedZ.N. HusseinM.A. MohamadE.A. STEN ameliorates VEGF gene expression by improving XBP1/mRNA-21/mRNA-330 signalling pathways in cisplatin-induced uterus injury in rats.J. Drug Deliv. Sci. Technol.20238710476010.1016/j.jddst.2023.104760
     [Google Scholar]
  25. ElgizawyH.A. AliA.A. HusseinM.A. Resveratrol: Isolation, and its nanostructured lipid carriers, inhibits cell proliferation, induces cell apoptosis in certain human cell lines carcinoma and exerts protective effect against paraquat-induced hepatotoxicity.J. Med. Food20212418910010.1089/jmf.2019.028632580673
     [Google Scholar]
  26. AliH. YesminR. SatterM.A. HabibR. YeasminT. Antioxidant and antineoplastic activities of methanolic extract of Kaempferia galanga Linn. Rhizome against Ehrlich ascites carcinoma cells.J. King Saud Univ. Sci.201830338639210.1016/j.jksus.2017.05.009
     [Google Scholar]
  27. AbalP. LouzaoM. AnteloA. Acute oral toxicity of tetrodotoxin in mice: determination of lethal dose 50 (LD50) and no observed adverse effect level (NOAEL).Toxins2017937510.3390/toxins903007528245573
     [Google Scholar]
  28. SayedH.M. SaidM.M. MorcosN.Y.S. El GawishM.A. IsmailA.F.M. Antitumor and radiosensitizing effects of zinc oxide-caffeic acid Nanoparticles against solid Ehrlich carcinoma in female mice.Integr. Cancer Ther.20212010.1177/15347354211021920
     [Google Scholar]
  29. OhkawaH. OhishiN. YagiK. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.Anal. Biochem.197995235135810.1016/0003‑2697(79)90738‑336810
     [Google Scholar]
  30. SedlakJ. LindsayR.H. Estimation of total protein bound and non-protein sulfhydryl groups in tissue with Ellmans reagent.Anal. Biochem.198525293298
     [Google Scholar]
  31. MisraH.P. FridovichI. The role of superoxide anion in the autooxidation of epinephrine anion in the autooxidation of epinephrine and a simple assay of superoxide dismutase.J. Biol. Chem.1972247317010.1016/S0021‑9258(19)45228‑94623845
     [Google Scholar]
  32. SinhaA.K. Colorimetric assay of catalase.Anal. Biochem.197247238939410.1016/0003‑2697(72)90132‑74556490
     [Google Scholar]
  33. AlturkistaniH.A. TashkandiF.M. MohammedsalehZ.M. Histological stains: A literature review and case study.Glob. J. Health Sci.201583727910.5539/gjhs.v8n3p7226493433
     [Google Scholar]
  34. BoshraS.A. HusseinM.A. Cranberry extract as a supplemented food in treatment of oxidative stress and breast cancer induced by N-Methyl-N-Nitrosourea in female virgin rats.Int. J. Phytomed.201682217227
     [Google Scholar]
  35. BancroftG.D. StevenA. Theory and practice of histological technique.4th edNew YorkChurchill Livingstone198399112
     [Google Scholar]
  36. SalahA. HusseinA. HassanS. HusseinM. BassiounyK. Green synthesis of RES-CMCS: A promising modulator of the glut-4/leptin signaling pathway in HFD-induced insulin resistance.Biomed. Res. Ther.2022975166517810.15419/bmrat.v9i7.753
     [Google Scholar]
  37. HusseinM.A. Anti-obesity, antiatherogenic, anti-diabetic and antioxidant activities of J. montana ethanolic formulation in obese diabetic rats fed high-fat diet.Free Radic. Antioxid.201111496010.5530/ax.2011.1.9
     [Google Scholar]
  38. El-gizawyH.A.E. HusseinM.A. Isolation, structure elucidation of ferulic and coumaric acids from fortunella japonica swingle leaves and their structure antioxidant activity relationship.Free Radic. Antioxid.201671233010.5530/fra.2017.1.4
     [Google Scholar]
  39. Mohammed AbdallaH.Jr Soad MohamedA.G. In vivo hepato-protective properties of purslane extracts on paracetamol-induced liver damage.Malays. J. Nutr.201016116117022691863
     [Google Scholar]
  40. El GizawyH.A.E.H. HusseinM.A. Abdel-SattarE. Biological activities, isolated compounds and HPLC profile of Verbascum nubicum.Pharm. Biol.201957148549710.1080/13880209.2019.164337831401911
     [Google Scholar]
  41. MosaadY.O. HusseinM.A. AteyyaH. Vanin 1 gene role in modulation of iNOS/MCP-1/TGF-β1 signaling pathway in obese diabetic patients.J. Inflamm. Res.2022156745675910.2147/JIR.S38650636540060
     [Google Scholar]
  42. El GizawyH.A. Abo-SalemH.M. AliA.A. HusseinM.A. Phenolic profiling and therapeutic potential of certain isolated compounds from parkia roxburghii against AChE activity as well as GABA A α5, GSK-3β, and p38α MAP-kinase genes.ACS Omega2021631204922051110.1021/acsomega.1c0234034395996
     [Google Scholar]
  43. WangY. MaJ. QiuT. TangM. ZhangX. DongW. In vitro and in vivo combinatorial anticancer effects of oxaliplatin- and resveratrol-loaded N,O-carboxymethyl chitosan nanoparticles against colorectal cancer.Eur. J. Pharm. Sci.202116310586410.1016/j.ejps.2021.10586433965502
     [Google Scholar]
  44. Abdel MaksoudH.A. ElharrifM.G. MahfouzM.K. OmniaM.A. AbdullahM.H. EltabeyM.E. Biochemical study on occupational inhalation of benzene vapours in petrol station.Respir. Med. Case Rep.20192710083610.1016/j.rmcr.2019.10083631008048
     [Google Scholar]
  45. GhorabM. IsmailZ. AbdalaM. Synthesis and biological activities of some novel triazoloquinazolines and triazinoquinazolines containing benzenesulfonamide moieties.Arzneimittelforschung2011602879510.1055/s‑0031‑129625420329657
     [Google Scholar]
  46. BainsM. KaurJ. AkhtarA. KuhadA. SahS.P. Anti-inflammatory effects of ellagic acid and vanillic acid against quinolinic acid-induced rat model of Huntington’s disease by targeting IKK-NF-κB pathway.Eur. J. Pharmacol.202293417531610.1016/j.ejphar.2022.17531636209926
     [Google Scholar]
  47. HusseinM.A. Synthesis of some novel triazoloquinazolines and triazinoquinazolines and their evaluation for anti-inflammatory activity.Med. Chem. Res.20122181876188610.1007/s00044‑011‑9707‑0
     [Google Scholar]
  48. HusseinM.A. Administration of exogenous surfactant and cytosolic phospholipase A2α inhibitors may Help COVID-19 infected patients with chronic diseases.Coronaviruses2021212e08092119222210.2174/2666796702666210311123323
     [Google Scholar]
  49. HusseinM.A. IsmailN.E.M. MohamedA.H. BorikR.M. AliA.A. MosaadY.O. Plasma phospholipids: A promising simple biochemical parameter to evaluate covid-19 infection severity.Bioinform. Biol. Insights20211510.1177/1177932221105589134840499
     [Google Scholar]
  50. BorikR.M. HusseinM.A. Synthesis, molecular docking, biological potentials and structure activity relationship of new quinazoline and quinazoline-4-one derivatives.Asian J. Chem.202133242343810.14233/ajchem.2021.23036
     [Google Scholar]
  51. GobbaN.A.E.K. Hussein AliA. El SharawyD.E. HusseinM.A. The potential hazardous effect of exposure to iron dust in Egyptian smoking and nonsmoking welders.Arch. Environ. Occup. Health201873318920210.1080/19338244.2017.131493028375782
     [Google Scholar]
  52. El-GizawyH.A. HusseinM.A. Fatty acids profile, nutritional values, anti-diabetic and antioxidant activity of the fixed oil of malvaparviflora growing in Egypt.Int. J. Phytomed.20157219230
     [Google Scholar]
  53. HusseinM.A. BorikR.M. NafieM.S. Abo-SalemH.M. BoshraS.A. MohamedZ.N. Structure activity relationship and molecular docking of some quinazolines bearing sulfamerazine moiety as new 3CLpro, cPLA2, sPLA2 inhibitors.Molecules20232816605210.3390/molecules2816605237630304
     [Google Scholar]
  54. BhosleS.M. HuilgolN.G. MishraK.P. Enhancement of radiation-induced oxidative stress and cytotoxicity in tumor cells by ellagic acid.Clin. Chim. Acta20053591-28910010.1016/j.cccn.2005.03.03715922998
     [Google Scholar]
  55. ChoiE.J. KimG.H. Apigenin induces apoptosis through a mitochondria/caspase-pathway in human breast cancer MDA-MB-453 cells.J. Clin. Biochem. Nutr.200944326026510.3164/jcbn.08‑23019430615
     [Google Scholar]
  56. a ShehataM.R. MohamedM.M.A. ShoukryM.M. HusseinM.A. HusseinF.M. Synthesis, characterization, equilibria, and biological activity of dimethyltin (IV) complex with 1, 4-piperazine.J. Coord. Chem.201568611011114
     [Google Scholar]
  57. b SinghM. SinghM. BhuiK. TyagiS. MahmoodZ. ShuklaY. Tea polyphenols induce apoptosis through mitochondrial pathway and by inhibiting nuclear factor kappa B and Akt activation in human cervical cancer cells.Oncol. Res.201119245257
     [Google Scholar]
  58. MikiY. SuzukiT. TazawaC. Aromatase localization in human breast cancer tissues: Possible interactions between intratumoral stromal and parenchymal cells.Cancer Res.20076783945395410.1158/0008‑5472.CAN‑06‑310517440110
     [Google Scholar]
  59. Abdul RahmanM.S. KanakarajanS. SelvarajR. Elucidation of the anticancer mechanism of durian fruit (durio zibethinus) pulp extract in human leukemia (HL-60) cancer cells.Nutrients20231510241710.3390/nu1510241737242300
     [Google Scholar]
  60. HusseinM.A. BorikR.M. A novel quinazoline-4-one derivatives as a promising cytokine inhibitors: Synthesis, molecular docking, and structure-activity relationship.Curr. Pharm. Biotechnol.20222391179120310.2174/138920102266621060117065034077343
     [Google Scholar]
  61. JomováK. HudecovaL. LauroP. A switch between antioxidant and prooxidant properties of the phenolic compounds myricetin, morin, 3′,4′-dihydroxyflavone, taxifolin and 4-hydroxy-coumarin in the presence of copper(II) ions: A spectroscopic, absorption titration and dna damage study.Molecules20192423433510.3390/molecules2423433531783535
     [Google Scholar]
  62. WangY. PanP. LiX. ZhuQ. HuangT. GeR.S. Food components and environmental chemicals of inhibiting human placental aromatase.Food Chem. Toxicol.2019128465310.1016/j.fct.2019.03.04330922969
     [Google Scholar]
  63. HeimerS. KnollG. Schulze-OsthoffK. EhrenschwenderM. Raptinal bypasses BAX, BAK, and BOK for mitochondrial outer membrane permeabilization and intrinsic apoptosis.Cell Death Dis.201910855610.1038/s41419‑019‑1790‑z31324752
     [Google Scholar]
  64. SaxenaM. DelgadoY. SharmaR.K. Inducing cell death in vitro in cancer cells by targeted delivery of cytochrome c via a transferrin conjugate.PLoS One2018134e019554210.1371/journal.pone.019554229649293
     [Google Scholar]
  65. ZhouZ. ZhuC. CaiZ. Betulin induces cytochrome c release and apoptosis in colon cancer cells via NOXA.Oncol. Lett.20181557319732710.3892/ol.2018.818329725447
     [Google Scholar]
  66. LiQ. LiB. LiQ. Exosomal miR-21-5p derived from gastric cancer promotes peritoneal metastasis via mesothelial-to-mesenchymal transition.Cell Death Dis.20189985410.1038/s41419‑018‑0928‑830154401
     [Google Scholar]
  67. LiJ. ShenJ. ZhaoY. Role of miR 181a 5p in cancer (Review).Int. J. Oncol.202363410810.3892/ijo.2023.555637539738
     [Google Scholar]
  68. GrassilliS. BertagnoloV. BrugnoliF. Mir-29b in breast cancer: A promising target for therapeutic approaches.Diagnostics2022129213910.3390/diagnostics1209213936140539
     [Google Scholar]
  69. SoofiyaniS.R. HosseiniK. SoleimanianA. An overview on the role of miR-451 in lung cancer: Diagnosis, therapy, and prognosis.MicroRNA202110318119010.2174/221153661066621091013082834514995
     [Google Scholar]
  70. SamsonovR. BurdakovV. ShtamT. Plasma exosomal miR-21 and miR-181a differentiates follicular from papillary thyroid cancer.Tumour Biol.2016379120111202110.1007/s13277‑016‑5065‑327164936
     [Google Scholar]
  71. SinghA. SinghA.K. GiriR. The role of microRNA-21 in the onset and progression of cancer.Future Med. Chem.202113211885190610.4155/fmc‑2021‑009634590501
     [Google Scholar]
  72. LiuW. LiuS.Y. HeY.B. MiR-451 suppresses proliferation, migration and promotes apoptosis of the human osteosarcoma by targeting macrophage migration inhibitory factor.Biomed. Pharmacother.20178762162710.1016/j.biopha.2016.12.12128086136
     [Google Scholar]
  73. CaiX. YinW. TangC. LuY. HeY. Molecular mechanism of microRNAs regulating apoptosis in osteosarcoma.Mol. Biol. Rep.20224976945695610.1007/s11033‑022‑07344‑x35474050
     [Google Scholar]
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RES-CMCNPs Enhance Antioxidant, Proinflammatory, and Sensitivity of Tumor Solids to γ-irradiation in EAC-Bearing Mice
Pharm. Nanotechnol. 13, 254 (2025); https://doi.org/10.2174/0122117385290497240324190453
/content/journals/pnt/10.2174/0122117385290497240324190453
/content/journals/pnt/10.2174/0122117385290497240324190453
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  • Article Type:     Research Article
Keyword(s): and NF-kB; carboxymethyl chitosan; caspase-3; P53; Res-CMCNPs; Resveratrol; γ-irradiation

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