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The carbohydrate derived non-ionic gemini surfactants have emerged as a result of our present day interest in environmentally compatible chemicals. The literature, including patents, describing the emerging area of carbohydrate derived non-ionic gemini surfactants is reviewed. The present review paper deals with synthesis, surface-active properties and biological properties of carbohydrate derived non-ionic gemini surfactants. The application of these surfactants in solubilization process, emulsification, nanotechnology, and gene and drug delivery systems has been described.