Synthesis, Molecular Docking and Biological Evaluation of Mannich Products Based on Thiophene Nucleus using Ionic Liquid

Background: We report here an efficient synthesis of new Mannich products 4(a-m) in excellent yields via multi-component reaction for the condensation of 5-bromothiophene- 2-carboxaldehyde (5-BT-2C), 2,4-dihydroxybenzophenone (2,4-DHBP) and primary/secondary amine derivative using Ethyl Ammonium Nitrate (EAN) as ionic liquid, which is reusable and plays an important role as a catalyst under 800C. Methods: All synthesized compounds were characterized by FT-IR, 1H-NMR, 13C-NMR spectra. All compounds were screened for their in vitro antibacterial, antifungal and antituberculosis activity, in which we found compounds 4b, 4c, 4d, 4h, 4i, 4j showing good to very good activity against all gram-positive and gram-negative bacteria. Results & Conclusion: Molecular docking studies showed that some of the synthesized compounds showed good docking interactions.