Synthesis and Evaluation of p-Nitrophenylβ-D-Glucopyranosiduronic Analogues as New Triggers for β-Glucuronidase Mediated Prodrug Mono- Therapy

Four new p-nitrophenyl β-D-glucuronide analogues, potentially useful as new triggers for β- glucuronidase mediated P.M.T. (Prodrug Mono-Therapy), have been prepared in a few steps. These compounds were tested for hydrolysis in the presence of either Escherichia Coli or bovine liver β-glucuronidase. The KM and Vmax values obtained for each analogue are reported.