A Cellular ELISA to Screen Lectin-Like Compounds for Cancer Cell Binding

C- P. Chang; W- C. Cheng; H- Y. Lei

doi:10.2174/1570180053765228

ISSN: 1570-1808
E-ISSN: 1875-628X

A Cellular ELISA to Screen Lectin-Like Compounds for Cancer Cell Binding
Authors: C- P. Chang¹, W- C. Cheng² and H- Y. Lei³
View Affiliations Hide Affiliations

¹ Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan. ² Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan. ³ Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Source: Letters in Drug Design & Discovery, Volume 2, Issue 3, May 2005, p. 182 - 188
DOI: https://doi.org/10.2174/1570180053765228
- Available online: 01 May 2005

Abstract

Lectins are carbohydrate-binding proteins that can modulate cellular activity through cell membrane glycoproteins. A cell-based binding was set up to screen the lectin-like substances in natural products. Lectins, after binding to tumor cells, are detected with a biotinylated carbohydrate-polyacrylamide complex by ELISA. Sugar specificity can also be determined within the assay.

Article metrics loading...

/content/journals/lddd/10.2174/1570180053765228

2005-05-01

2025-06-12

From This Site

/content/journals/lddd/10.2174/1570180053765228

dcterms_title,dcterms_subject,pub_keyword

-contentType:Contributor -contentType:Concept -contentType:Institution

10

5

Full text loading...

/content/journals/lddd/10.2174/1570180053765228

Article Type: Review Article

Keyword(s): apoptosis; elisa; lectin; mitogenic

A Cellular ELISA to Screen Lectin-Like Compounds for Cancer Cell Binding

Abstract

From This Site

Most Read This Month

Most Cited Most Cited RSS feed

Discovery, Structural Determination and Anticancer Activities of Lactucinlike Guaianolides

Thermodynamic Aspects of Cancer: Possible Role of Negative Entropy in Tumor Growth, its Relation to Kinetic and Genetic Resistance

Immunomodulatory Properties of Synthetic Imidazolone Derivatives

Design and Prediction of ADME/Tox Properties of Novel Magnolol Derivatives as Anticancer Agents for NSCLC Using 3D-QSAR, Molecular Docking, MOLCAD and MM-GBSA Studies

Redox Active Iron at the Center of Oxidative Stress in Alzheimer Disease

Identifying Protein Binding Sites and Optimal Ligands

Phenyldihydroxypyrimidines as HCV NS5B RNA Dependent RNA Polymerase Inhibitors. Part I: Amides and Ureas

Phenyldihydroxypyrimidines as HCV NS5B RNA Dependent RNA Polymerase Inhibitors. Part II: Sulfonamides

Selection of a Catalytically Active Enzyme In Vivo Via Phage Display of its Product

A Comprehensive Review of the Advancement in Omic Technologies in the Field of Drug Discovery and Development