A Cellular ELISA to Screen Lectin-Like Compounds for Cancer Cell Binding

C- P. Chang; W- C. Cheng; H- Y. Lei

doi:10.2174/1570180053765228

ISSN: 1570-1808
E-ISSN: 1875-628X

A Cellular ELISA to Screen Lectin-Like Compounds for Cancer Cell Binding
Authors: C- P. Chang¹, W- C. Cheng² and H- Y. Lei³
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¹ Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan. ² Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan. ³ Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Source: Letters in Drug Design & Discovery, Volume 2, Issue 3, May 2005, p. 182 - 188
DOI: https://doi.org/10.2174/1570180053765228
- Available online: 01 May 2005

Abstract

Lectins are carbohydrate-binding proteins that can modulate cellular activity through cell membrane glycoproteins. A cell-based binding was set up to screen the lectin-like substances in natural products. Lectins, after binding to tumor cells, are detected with a biotinylated carbohydrate-polyacrylamide complex by ELISA. Sugar specificity can also be determined within the assay.

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2005-05-01

2025-05-27

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Article Type: Review Article

Keyword(s): apoptosis; elisa; lectin; mitogenic

A Cellular ELISA to Screen Lectin-Like Compounds for Cancer Cell Binding

Abstract

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