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Volume 21, Issue 17
  • ISSN: 1570-1808
  • E-ISSN: 1875-628X

Abstract

Objectives

Intra-abdominal adhesions are the most frequently occurring postoperative complication following abdominopelvic surgery. Peritoneal adhesion formation can lead to infertility, chronic pelvic pain, and intestinal obstruction. Several studies have shown the potential role of metformin in reducing inflammation. Here, we explored the therapeutic potency of Metformin against postsurgical adhesion band formation.

Methods

We adopted Animal Research: Reporting of Experiments (ARRIVE) guidelines for the experimental protocol. Peritoneal adhesions were examined macroscopically. Metformin (100 mg/kg) was administered intraperitoneally in male albino Wistar rats. After 9 days, macroscopic evidence and score of fibrotic bands based on scaling methods were performed and evaluated by the Nair and Leach scoring system. Moreover, the anti-inflammatory and antifibrosis effects were investigated by using hematoxylin and eosin, Masson's trichrome staining, the enzyme-linked immunosorbent assay (ELISA), and reverse transcription polymerase chain reaction (RT-PCR).

Results

Metformin inhibited the stabilization of adhesion bands and appeared to elicit anti-inflammatory responses by attenuating submucosal edema, suppressing proinflammatory cytokines, decreasing proinflammatory cell infiltration, and inhibiting oxidative stress at the site of peritoneal surgery. We also showed that metformin prevents fibrotic adhesion band formation by reducing excessive collagen deposition and suppression of profibrotic gene expression at the peritoneum adhesion tissues modulation of Col 1A1/A3.

Conclusion

These results supported the potential application of metformin in preventing postsurgical adhesion band formation by inhibiting key pathologic responses of inflammation and fibrosis in patients post-surgery.

© 2024 Bentham Science Publishers
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2024-09-30
2025-04-23

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The Therapeutic Potential of Metformin for Attenuating Postsurgical Adhesion Band Formation
Letters in Drug Design & Discovery 21, 4037 (2024); https://doi.org/10.2174/0115701808301517240827092315
/content/journals/lddd/10.2174/0115701808301517240827092315
/content/journals/lddd/10.2174/0115701808301517240827092315
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  • Article Type:     Research Article
Keyword(s): fibrosis; inflammatory response; Intra-abdominal adhesion; metformin; RT-PCR; transforming growth factor beta

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