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2000
Volume 20, Issue 6
  • ISSN: 1871-5265
  • E-ISSN: 2212-3989

Abstract

Background: Although a majority of patients with cutaneous leishmaniasis (CL) are healed with Glucantime chemotherapy, the increased drug resistance rate following its application is a concern. In this study, crude venom of cobra snakes was used for the first time as an assembled combination of bioactive pharmaceutical components on (L. major) standard strain. Objective: To evaluate the efficacy of the crude venom of Iranian cobra snakes on standard strain . Methods: Five concentrations (1.25, 2.5, 5, 10 and 20 μg/mL) of venom were added to Leishmania major cultures at 24, 48 and 72 h. . The apoptosis was determined by flow cytometry, while IC was determined by counting parasites compared to that of glucantime. Each test was conducted in triplicate. Results: After the exposure of the venom for 72 h, IC value was 0.36 μg/mL and 14.12 μg/mL for promastigotes and amastigotes, respectively. MTT valuation clarified 1.01% promastigotes viability. Furthermore, the results indicated that the venom of induced early and late apoptosis in parasites. Conclusions: The venom of revealed remarkable anti-leishmanial effects as a novel anti-parasitic alternative. Thus, the bioactive and effective fractions of this venom may be considered as anti-leishmanial candidates in future studies.

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/content/journals/iddt/10.2174/1871526520666200106121839
2020-12-01
2025-05-08
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/content/journals/iddt/10.2174/1871526520666200106121839
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  • Article Type:
    Research Article
Keyword(s): apoptosis; IC50; Iranian cobra; Leishmania major; MTT; Naja naja oxiana
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