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Volume 24, Issue 22
  • ISSN: 1568-0266
  • E-ISSN: 1873-4294
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Abstract

Background

The synthesis of nanoparticles using the principle of green chemistry has achieved huge potential in nanomedicine. Here, we report the synthesis of silver nanoparticles (AgNPs) employing garlic essential oil (GEO) due to wide applications of GEO in the biomedical and pharmaceutical industry.

Objective

This study aimed to synthesise garlic essential oil-assisted silver nanoparticles and present their antimicrobial and antibiofilm activities with mechanistic assessment.

Methods

Initially, the formulation of AgNPs was confirmed using different optical techniques, such as XRD, FT-IR, DLS, zeta potential, SEM, and EDX analysis, which confirmed the formulation of well-dispersed, stable, and spherical AgNPs. The antimicrobial and antibiofilm activity of GEO-assisted AgNPs was evaluated against a spectrum of pathogenic microorganisms, such as Gram-positive ( and ) and Gram-negative ( and ) bacteria.

Results

The AgNPs exhibited remarkable antimicrobial and anti-biofilm activity against all tested strains. The mechanism behind the antimicrobial activity of AgNPs was explored by estimating the amount of reactive oxygen species (ROS) generated due to the interaction of AgNP with bacterial cells and observing the morphological changes of bacteria upon AgNP interaction.

Conclusion

The findings of this study concluded that ROS generation due to the interaction of AgNPs with bacterial cells put stress on bacterial membranes, altering the morphology of bacteria, exhibiting remarkable antimicrobial activity, and preventing biofilm formation.

© 2024 Bentham Science Publishers
© 2024 The Author(s). Published by Bentham Science Publisher. This is an open access article published under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/legalcode
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2024-09-01
2024-11-12

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Formulation of Garlic Essential Oil-assisted Silver Nanoparticles and Mechanistic Evaluation of their Antimicrobial Activity against a Spectrum of Pathogenic Microorganisms
Curr. Top. Med. Chem. 24, 2000 (2024); https://doi.org/10.2174/0115680266322180240712055727
/content/journals/ctmc/10.2174/0115680266322180240712055727
/content/journals/ctmc/10.2174/0115680266322180240712055727
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  • Article Type:     Research Article
Keyword(s): Antibiofilm activity; Antimicrobial activity; Garlic essential oil; Green synthesis; Reactive oxygen species; Silver nanoparticles

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