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Volume 20, Issue 3
  • ISSN: 1574-888X
  • E-ISSN: 2212-3946

Abstract

Background

maturation has been considered an approach to mature oocytes derived from women with polycystic ovary syndrome (PCOS). It is suggested that the IVM of oocytes may benefit from mesenchymal stem cells derived conditioned medium (CM-MSC).

Objective

The purpose of this study was to determine the efficacy of a cocktail of menstrual blood stem cell (MenSCs)-derived secretome, along with follicular fluid and melatonin, in oocyte maturation and embryo development in PCOS.

Methods

Four hundred left germinal vesicle oocytes were collected from 100 PCOS patients and randomly divided into four treatment groups: 1) control, 2) secretome, 3) follicular fluid, and 4) melatonin. Oocyte maturation, fertilization rate, and embryo development were monitored, as well as the expression levels of oocyte-secreted factors (GDF9- BMP15), oocyte maturation (MPK3), and apoptosis (BAX-Bcl2).

Results

The rate of oocyte maturation increased in all test groups, but only the results for the SEC group were significant (P= 0.032). There were no significant differences in oocyte fertilization and embryo yield among groups. However, the quality of embryos significantly increased in the melatonin group compared to the control. Cytoplasmic maturation was confirmed by the expression of oocyte maturation-related genes using Real-time PCR. Additionally, the expression level of BCL-2 was significantly higher in the SEC-FF-MEL group than in the control group ( ≤ 0.01).

Conclusion

Enrichment of IVM media using MenSCs-secretome, particularly along with melatonin, could be an effective strategy to improve oocyte maturation and embryo development in PCOS.

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2025-05-03

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/content/journals/cscr/10.2174/011574888X298902240523103352
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Role of Menstrual Blood Stem Cell-Derived Secretome, Follicular Fluid, and Melatonin in Oocyte Maturation and Embryo Development in Polycystic Ovary Syndrome
Curr Stem Cell Res Ther 20, 291 (2025); https://doi.org/10.2174/011574888X298902240523103352
/content/journals/cscr/10.2174/011574888X298902240523103352
/content/journals/cscr/10.2174/011574888X298902240523103352
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  • Article Type:     Research Article
Keyword(s): GV-oocyte; IVM; melatonin; Menstrual blood stem cells; PCO; secretome

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