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Volume 26, Issue 4
  • ISSN: 1389-2010
  • E-ISSN: 1873-4316

Abstract

Background

The study aimed to assess the antioxidant and wound healing properties of essential oil (UDEO) through a comprehensive evaluation involving analyses. The phytochemistry of UDEO was also investigated to identify trace compounds crucial.

Methods

Various injection methods of the multimode inlet (MMI) in chromatography were investigated to attain lower instrumental detection limits. Subsequently, studies were employed to delve deeper into the potential biological activities of the identified compounds. Standard antioxidative tests, encompassing ABTS•+ and TAC, were performed. tests centered on wound healing were implemented using rat models. The rats were randomly allocated to four groups: saline solution, vaseline vehicle, cytol centella, and 5% UDEO ointment. Wound healing progress was evaluated through a chromatic study.

Results

Gas chromatography combined with triple quadrupole mass spectrometry (GC-MS/MS) analysis revealed the presence of 97 thermolabile compounds in UDEO. Subsequent studies unveiled the potential of identified compounds to inhibit COX-2, TNF-α, and IL-6, suggesting a possible enhancement of anti-inflammatory responses and healing processes. tests elucidated the notable antioxidant capacity of UDEO, a finding reinforced by wound healing data, revealing a substantial closure rate of 89% following the topical application of UDEO. Notably, fibrinogen and C-reactive protein (CRP) levels were significantly reduced, indicating minimized oxidative stress damage compared to control. Additionally, UDEO exhibited an increase in antioxidant enzyme activities compared to control.

Conclusion

The study concludes that UDEO possesses significant antioxidant and wound-healing properties, supported by its rich phytochemical composition. The findings suggest its potential application in therapeutic interventions for oxidative stress and inflammatory conditions.

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GC-MS/MS Analysis and Wound Repair Potential of Urtica dioica Essential Oil: In silico Modeling and In vivo Study in Rats
Current Pharmaceutical Biotechnology 26, 591 (2025); https://doi.org/10.2174/0113892010304346240619061848
/content/journals/cpb/10.2174/0113892010304346240619061848
/content/journals/cpb/10.2174/0113892010304346240619061848
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  • Article Type:     Research Article
Keyword(s): biochemical parameters; excision wound; GCMS/MS analysis; healing activity; in silico modeling; U. dioica

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