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Volume 26, Issue 1
  • ISSN: 1389-2010
  • E-ISSN: 1873-4316

Abstract

Background

Breast Cancer (BC) is a female malignancy with a high mortality rate. Novel diagnostic and prognostic biomarkers are valuable for reducing BC mortality. Our study is designed to undrape the precise role of the LINC00466/miR-4731-5p/EPHA2 axis in BC.

Methods

The Cancer Genome Atlas (TCGA) sequencing dataset was utilized to compare the levels of LINC00466. The levels of LINC00466, miR-4731-5p, and EPHA2 were tested by qRT-PCR. Cell proliferation and cycle were detected by CCK-8 assay and flow cytometer. role of LINC00466 was tested by Xenograft nude models. Binding sites were predicted by TargetScan and Starbase. The binding relationship was employed by Dual-luciferase reporter gene assay and RNA pull-down assay.

Results

LINC00466 was increased in human breast cancer tissues. LINC00466 was negatively associated with miR-4731-5p and positively correlated with EPHA2 in human breast cancer tissues. Down-regulation of LINC00466 suppressed the proliferation and arrested the cell cycle of breast cancer cells, and inhibited tumor growth .

Conclusion

LINC00466 promoted BC development mediating the miR-4731-5p/EPHA2 axis, which has the potential value as a promising therapeutic target in BC.

© 2025 Bentham Science Publishers
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2024-05-09
2025-04-02

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/content/journals/cpb/10.2174/0113892010290582240419051056
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LncRNA LINC00466 Promotes the Progression of Breast Cancer via miR-4731-5p/EPHA2 Pathway
Current Pharmaceutical Biotechnology 26, 120 (2025); https://doi.org/10.2174/0113892010290582240419051056
/content/journals/cpb/10.2174/0113892010290582240419051056
/content/journals/cpb/10.2174/0113892010290582240419051056
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  • Article Type:     Research Article
Keyword(s): biomarkers; Breast cancer; EPHA2; LINC00466; miR-4731-5p; Xenograft nude models

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