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Volume 26, Issue 3
  • ISSN: 1389-2010
  • E-ISSN: 1873-4316

Abstract

Introduction

TP508 is a thrombin peptide that participates in the inflammatory response and wound healing. Its role in the molecular mechanism of distraction osteogenesis remains unclear. This study established a tibia distraction osteogenesis (DO) model in rats and investigated the role and mechanism of TP508 in bone regeneration during DO.

Methods

Micro-computed tomography (Micro-CT) and hematoxylin-eosin (HE) staining were used to track osteogenesis. Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to measure the expression of osteoblast-related factors, Wnt/β-catenin signaling-related proteins and genes. Immunohistochemistry was used to measure the expression of β-catenin in the cytoplasm and nucleus.

Results

TP508 accelerated bone regeneration increased the expression of the osteoblast-related factors Alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), and osteocalcin (OCN). After the Wnt signaling was inhibited by LGK974, the expression of osteoblast-related factors was downregulated, leading to a decrease in bone regeneration ability. More importantly, TP508 upregulated β-catenin and its target CYCLIN-D1 and could reverse the decreased osteogenic ability caused by LGK974.

Conclusion

In conclusion, TP508 promotes bone regeneration in DO by activating the Wnt/β-catenin signaling pathway.

© 2025 Bentham Science Publishers
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2024-03-11
2025-04-24

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TP508 Promotes Bone Regeneration on Distraction Osteogenesis via the Activation of Wnt/β-catenin Signaling Pathway
Current Pharmaceutical Biotechnology 26, 402 (2025); https://doi.org/10.2174/0113892010289575240306033011
/content/journals/cpb/10.2174/0113892010289575240306033011
/content/journals/cpb/10.2174/0113892010289575240306033011
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  • Article Type:     Research Article
Keyword(s): Distraction osteogenesis; molecular mechanism; thrombin peptide; tissue engineering; TP508; Wnt signaling

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