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2000
Volume 20, Issue 1
  • ISSN: 1573-4129
  • E-ISSN: 1875-676X

Abstract

Objective: This study aimed to establish a simple, reliable, and sensitive method for detecting ketamine, fluoroketamine, and their metabolites in urine using UPLC-MS/MS. Methods: The chromatographic separation was performed on UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) at a column temperature of 40°C. The mobile phase consisted of 0.1% formic acid aqueous solution and acetonitrile, with a flow rate set at 0.4 mL/min, following a specific elution procedure. A urine sample was treated with acetonitrile, and midazolam was used as an internal standard. Multiple reaction monitoring was used for quantitative analysis. Results: Ketamine, fluoroketamine, norketamine, and 2-norfluoro-ketamine exhibited linearity in urine (r>0.99) within the concentration range of 5-2000 ng/mL. Intra-day and inter-day precisions were 9% or less and 12% or less, respectively. The accuracy ranged from 92 to 107%. Mean recoveries were above 76%. The measured matrix effect was between 85 and 104%. Conclusion: This simple, reliable, and sensitive PLC-MS/MS method was successfully developed to determine ketamine, fluoroketamine, and their metabolite in rat urine.

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/content/journals/cpa/10.2174/0115734129280521240110094134
2024-01-01
2025-07-07
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/content/journals/cpa/10.2174/0115734129280521240110094134
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  • Article Type:
    Research Article
Keyword(s): 2-norfluoro-ketamine; fluoroketamine; Ketamine; norketamine; rat urine; UPLC-MS/MS
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