Current Proteomics - Volume 4, Issue 2, 2007

Proteins are the major players in most processes of living cells and study of the proteome has great relevance to investigations on cells and organisms at the molecular level. 2-DE is a core and powerful proteomic technique to study protein expression and function in living organisms and it allows a fast overview of changes that occurred in cells at the proteome level. Although data on DNA sequences from large scale geno Read More

Phytopathogenic fungi are organisms responsible for several plant diseases in different crops around the world, causing very important economic losses to the farmers. Fungi have a complicated life cycle, normally with asexual and sexual reproduction that involves the formation of different reproductive structures. Moreover, during plant disease, fungi produce different components that are essential to complete the infectio Read More

Global characterisation of proteins and the comparison between proteomes is providing new insights into general biological structures and processes, as well as between physiological and pathological conditions. During both embryonic development and adult life, brain cavities and ventricles are filled with a complex protein-rich fluid, the cerebrospinal fluid (CSF). In the last few years, it has been suggested that adult Read More

The bee venom is used for treating a wide variety of conditions from acute tendonitis to chronic back pain to rheumatoid arthritis (RA). The major treatment is gene therapy or recombinant DNA vaccines involved in targeting multiple antigenic components to direct and empower the immune system to protect the host from infection. Limitations of therapy for the treatment of patients suffering from various adverse reaction a Read More

We have produced a safe and convenient “fluorescence imager” to observe the protein bands and spots in the polyacrylamide gel stained by the fluorescent stain. Coomassie brilliant blue (CBB) and the argentation or silver stain method have been used usually for this staining. The staining of the gel using a fluorescent stain, such as SYPRO Ruby was developed recently. This fluorescent stain has more advantages compared to Read More