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Nano-sized silver powders coated with sodium oleate (NanoAg@C18) was prepared by a simple wet chemical route and characterized by FT-IR, DTA-TG, TEM, SAED and EDS. The main objective of this research was to successfully employ the NanoAg@C18 for the inhibition of bacterial and fungal biofilms in vitro development on catheter sections with a film-coated surface. The lower value of the relative microbial biomass formed on the surface of the modified prosthetic devices demonstrates the anti-biofilm efficiency of the NanoAg@C18 coating and the potential use of this nanosytem for obtaining improved medical devices.