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Volume 21, Issue 3
  • ISSN: 1573-4013
  • E-ISSN: 2212-3881

Abstract

Background

Postbiotics, also called biogenics, metabiotics, or Cell-Free Supernatants (CFS), are soluble compounds produced by live probiotic microorganisms or released after the lysis of probiotics that could provide beneficial health effects to the host.

Objective

This study was conducted with the aim of investigating the antimicrobial, antibiofilm, antioxidant, and cytotoxic effects of postbiotics derived from .

Methods

In this study, the antimicrobial properties of the postbiotic of were investigated in different experimental settings, and pasteurized milk. The antioxidant effect of postbiotic was also evaluated by -diphenyl-1-picrylhydrazyl (DPPH) and 2, 20-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) method. Furthermore, the chemical composition of the postbiotics was identified gas chromatography-mass spectrometry. The cytotoxic effects of the compounds were investigated using a human normal cell line.

Results and Discussion

According to the results, postbiotics had aconcentration-dependent antimicrobial effect, and the inhibitory effect increased with increasing concentrations. The antimicrobial activity was mainly linked with lactic acid and laurostearic acid. The Minimum Inhibitory Concentration (MIC) of the prepared postbiotic was determined to be 100 mg/ml. The lowest Minimum Effective Concentration (MEC) of postbiotics significantly differed in the food matrix, and a low MEC (minimum effective concentration) index (150 mg/ml) was detected for postbiotic of .

Conclusion

Therefore, the prepared postbiotic should be subjected to more in-depth analysis to examine its suitability as a food additive.

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2024-12-27

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Characterization and Antimicrobial Activity of Postbiotic from Lactobacillus Acidophilus LA5 on Staphylococcus Aureus in Food Model and In vitro
Curr. Nutr. Food Sci. 21, 379 (2025); https://doi.org/10.2174/0115734013305717240718104323
/content/journals/cnf/10.2174/0115734013305717240718104323
/content/journals/cnf/10.2174/0115734013305717240718104323
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  • Article Type:     Research Article
Keyword(s): antimicrobial activity; chemical composition; chromatography-mass spectrometry; lactic acid; Postbiotics; probiotic

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