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Volume 21, Issue 1
  • ISSN: 1573-4137
  • E-ISSN: 1875-6786

Abstract

Background

The use of targeted therapy has been increasing for cancer treatment. The aim of this study is to investigate chitosan-based ricin-Herceptin (rh) immunotoxin on breast cancer cell lines.

Methods

The gene construct encoding immunotoxin was designed, cloned, and expressed in BL21 (DE3). The expressed proteins were isolated by the nickel-nitrilotriacetic acid column and were analyzed by the Western-blotting. The cytotoxicity of immunotoxin was assayed on breast cell line MCF-7 and using MTT assay at 24 and 48 h treatment.

Results

The immunotoxins extrication rate, size, loading percentage, and electric charge of nanoparticles were reported appropriately as 78%, 151.5 nm, 83.53%, and +11.1 mV, respectively. The encapsulated immunotoxins led to the death of 70% and 78% of MCF-7 cells at 24 and 48 h treatment, respectively. The noncapsulated counterparts at equal doses killed 53% and 62% of cancer cells at the same time points.

Conclusion

The chitosan-immunotoxins impose potential cytotoxic effects on cancer cells.

© 2025 Bentham Science Publishers
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2025-01-01
2024-11-26

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/content/journals/cnano/10.2174/0115734137278545240102055626
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The Cytotoxicity Effect of Chitosan-Encapsulated Ricin-Herceptin Immunotoxin Nanoparticles on Breast Cancer Cell Lines
Curr. Nanosci. 21, 140 (2025); https://doi.org/10.2174/0115734137278545240102055626
/content/journals/cnano/10.2174/0115734137278545240102055626
/content/journals/cnano/10.2174/0115734137278545240102055626
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  • Article Type:     Research Article
Keyword(s): breast cancer; HER2; Herceptin; nanoparticles; ricin; targeted therapy

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