Skip to content
2000
  1. Home
  2. A-Z Publications
  3. Current Molecular Pharmacology
  4. Volume 17, Issue 1
  5. Article
Volume 17, Issue 1
  • ISSN: 1874-4672
  • E-ISSN: 1874-4702
file format text download EPUB
file format pdf download PDF
side by side viewer icon HTML

Abstract

Background

Over the last several decades, the AcrAB and OqxAB efflux pumps have been found to cause multidrug resistance (MDR) in various bacteria, most notably . Antibiotic resistance surges with increased expression of the and efflux pumps.

Methods

In accordance with CLSI guidelines, a disk diffusion test was carried out using 50 isolates obtained from various clinical samples. CT was computed in treated samples and compared to a susceptible ciprofloxacin strain (A111). The final finding is presented as the fold change in the target gene's expression in treated samples relative to a control sample (A111), normalized to a reference gene. As ∆∆CT = 0 and 2 to the power of 0 = 1, relative gene expression for reference samples is often set to 1

Results

The highest rates of resistance were recognized with cefotaxime (100%), cefuroxime (100%), cefepime (100%), levofloxacin (98%), trimethoprim-sulfamethoxazole (80%), and gentamicin (72%), whereas imipenem (34%) had the lowest rates. Overexpression of and , and , regulators , , and were greater in ciprofloxacin-resistant isolates compared to the reference strain (strain A111). There was also a moderate connection between ciprofloxacin MIC and gene expression and a moderate connection between ciprofloxacin MIC and gene expression.

Conclusion

This work provides a deeper knowledge of the role of efflux pump genes, particularly and , as well as transcriptional regulators , soxS, and , in bacterial resistance to ciprofloxacin.

© 2024 The Author(s). Published by Bentham Science Publisher.
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Loading

Article metrics loading...

/content/journals/cmp/10.2174/1874467217666230331081434
2023-07-13
2024-11-23

  • From This Site

    /content/journals/cmp/10.2174/1874467217666230331081434
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

/deliver/fulltext/cmp/17/1/CMP-17-E310323215266.html?itemId=/content/journals/cmp/10.2174/1874467217666230331081434&mimeType=html&fmt=ahah

References

  1. AlMatarM. AlbarriO. MakkyE.A. VarI. KöksalF. An overview of the activities of cefiderocol against sensitive and multidrug-resistant (MDR) bacteria.Mini Rev. Med. Chem.202020181908191610.2174/138955752066620081821140532811410
     [Google Scholar]
  2. AlMatarM. AlbarriO. MakkyE.A. VarI. KöksalF. A glance on the role of bacterial siderophore from the perspectives of medical and biotechnological approaches.Curr. Drug Targets202021131326134310.2174/138945012166620062119301832564749
     [Google Scholar]
  3. GiskeC.G. MonnetD.L. CarsO. CarmeliY. Clinical and economic impact of common multidrug-resistant gram-negative bacilli.Antimicrob. Agents Chemother.200852381382110.1128/AAC.01169‑0718070961
     [Google Scholar]
  4. OsmanA. IşılV. FatihK. Microbial siderophores: Potential medicinal applications of the siderophores.J. Biotechnol. Sci. Res201963240
     [Google Scholar]
  5. HansenL.H. JensenL.B. SørensenH.I. SørensenS.J. Substrate specificity of the OqxAB multidrug resistance pump in Escherichia coli and selected enteric bacteria.J. Antimicrob. Chemother.200760114514710.1093/jac/dkm16717526501
     [Google Scholar]
  6. NormanA. HansenL.H. SheQ. SørensenS.J. Nucleotide sequence of pOLA52: A conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux.Plasmid2008601597410.1016/j.plasmid.2008.03.00318440636
     [Google Scholar]
  7. Rodríguez-MartínezJ.M. Díaz de AlbaP. BrialesA. MachucaJ. LossaM. Fernández-CuencaF. RodríguezB.J. Martínez-MartínezL. PascualA. Contribution of OqxAB efflux pumps to quinolone resistance in extended-spectrum- -lactamase-producing Klebsiella pneumoniae.J. Antimicrob. Chemother.2013681687310.1093/jac/dks37723011289
     [Google Scholar]
  8. SzaboO. KocsisB. SzaboN. KristofK. SzaboD. Contribution of OqxAB efflux pump in selection of fluoroquinolone-resistant Klebsiella pneumoniae.Can. J. Infect. Dis. Med. Microbiol.201820181510.1155/2018/427163830344799
     [Google Scholar]
  9. WongM.H.Y. ChanE.W.C. ChenS. Evolution and dissemination of OqxAB-like efflux pumps, an emerging quinolone resistance determinant among members of Enterobacteriaceae.Antimicrob. Agents Chemother.20155963290329710.1128/AAC.00310‑1525801572
     [Google Scholar]
  10. HasdemirU.O. ChevalierJ. NordmannP. PagèsJ.M. Detection and prevalence of active drug efflux mechanism in various multidrug-resistant Klebsiella pneumoniae strains from Turkey.J. Clin. Microbiol.20044262701270610.1128/JCM.42.6.2701‑2706.200415184455
     [Google Scholar]
  11. MazzariolA. TokueY. KanegawaT.M. CornagliaG. NikaidoH. High-level fluoroquinolone-resistant clinical isolates of Escherichia coli overproduce multidrug efflux protein AcrA.Antimicrob. Agents Chemother.200044123441344310.1128/AAC.44.12.3441‑3443.200011083655
     [Google Scholar]
  12. MahonC.R. LehmanD.C. ManuselisG. Textbook of diagnostic microbiology-e-book.Elsevier Health Sciences2018
     [Google Scholar]
  13. DehnamakiM. GhaneM. BabaeekhouL. Detection of OqxAB and QepA efflux pumps and their association with antibiotic resistance in Klebsiella pneumoniae isolated from urinary tract infection.Int. J. Infect202074e10739710.5812/iji.107397
     [Google Scholar]
  14. SchwalbeR. Steele-MooreL. GoodwinA.C. Antimicrobial susceptibility testing protocols.Crc Press200710.1201/9781420014495
     [Google Scholar]
  15. DufourP. ColonM. The tetrazolium reduction method for assessing the viability of individual bacterial cells in aquatic environments: Improvements, performance and applications.Hydrobiologia1992232321121810.1007/BF00013706
     [Google Scholar]
  16. RazaviS. MirnejadR. BabapourE. Involvement of AcrAB and OqxAB efflux pumps in antimicrobial resistance of clinical isolates of Klebsiella pneumonia.Appl. Biotechnol. Rep.20207251257
     [Google Scholar]
  17. WandM.E. DarbyE.M. BlairJ.M.A. SuttonJ.M. Contribution of the efflux pump AcrAB-TolC to the tolerance of chlorhexidine and other biocides in Klebsiella spp.J. Med. Microbiol.202271300149610.1099/jmm.0.00149635324422
     [Google Scholar]
  18. AlMatarM. VarI. KayarB. KöksalF. Differential expression of resistant and efflux pump genes in MDR-TB isolates.Endocr Metab Immune Disord Drug Targets202020227128710.2174/187153031966619100915383431595857
     [Google Scholar]
  19. AlbarriO. AlMatarM. ÖcalM.M. KöksalF. Overexpression of efflux pumps AcrAB and OqxAB contributes to ciprofloxacin resistance in clinical isolates of K. pneumonia.Curr. Protein Pept. Sci.202223535636810.2174/138920372366622063016292035786184
     [Google Scholar]
  20. ZhengJ. LinZ. SunX. LinW. ChenZ. WuY. QiG. DengQ. QuD. YuZ. Overexpression of OqxAB and MacAB efflux pumps contributes to eravacycline resistance and heteroresistance in clinical isolates of Klebsiella pneumoniae .Emerg. Microbes Infect.20187111110.1038/s41426‑018‑0141‑y30068997
     [Google Scholar]
  21. LivakK. J. SchmittgenT. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method.Methods200125440240810.1006/meth.2001.126211846609
     [Google Scholar]
  22. Mohd AsriN.A. AhmadS. MohamudR. Mohd HanafiN. Mohd ZaidiN.F. IrekeolaA.A. ShuebR.H. YeeL.C. Mohd NoorN. MustafaF.H. YeanC.Y. YusofN.Y. Global prevalence of nosocomial multidrug-resistant Klebsiella pneumoniae: a systematic review and meta-analysis.Antibiotics20211012150810.3390/antibiotics1012150834943720
     [Google Scholar]
  23. Ashayeri-PanahM. FeizabadiM.M. EftekharF. Correlation of multi-drug resistance, integron and blaESBL gene carriage with genetic fingerprints of extended-spectrum β-lactamase producing Klebsiella pneumoniae.Jundishapur J. Microbiol.201472e874710.5812/jjm.874725147670
     [Google Scholar]
  24. PoirelL. ÖzdamarM. Ocampo-SosaA.A. TürkogluS. OzerU.G. NordmannP. NDM-1-producing Klebsiella pneumoniae now in Turkey.Antimicrob. Agents Chemother.20125652784278510.1128/AAC.00150‑1222391536
     [Google Scholar]
  25. Yousefi MashoufR. AlijaniP. SaidijamM. AlikhaniM.Y. RashidiH. Study of antibiotic resistance pattern and phenotypic detection of ESBLs in Klebsiella pneumoniae strains isolated from clinical samples and determination of minimum inhibitory concentrations of imipenem and ceftazidim antibiotics.Avicenna J. Med. Biotechnol.201420295302
     [Google Scholar]
  26. HashemiA. FallahF. TaherpourA. GoudarziH. ErfanimaneshS. TakiE. Evaluation of genetic pattern and determination of oqxA gene expression levels among clinical isolates of Klebsiella pneumoniae strains.J. Mazandaran Univ. Med. Sci.2014244861
     [Google Scholar]
  27. LiuB.T. WangX.M. LiaoX.P. SunJ. ZhuH.Q. ChenX.Y. LiuY.H. Plasmid-mediated quinolone resistance determinants oqxAB and aac(6′)-Ib-cr and extended-spectrum -lactamase gene blaCTX-M-24 co-located on the same plasmid in one Escherichia coli strain from China.J. Antimicrob. Chemother.20116671638163910.1093/jac/dkr17221546384
     [Google Scholar]
  28. MahamoudA. ChevalierJ. Alibert-FrancoS. KernW.V. PagèsJ.M. Antibiotic efflux pumps in Gram-negative bacteria: the inhibitor response strategy.J. Antimicrob. Chemother.20075961223122910.1093/jac/dkl49317229832
     [Google Scholar]
  29. MeletisG. ExindariM. VavatsiN. SofianouD. DizaE. Mechanisms responsible for the emergence of carbapenem resistance in Pseudomonas aeruginosa.Hippokratia201216430330723935307
     [Google Scholar]
  30. WasfiR. ElkhatibW.F. AshourH.M. Molecular typing and virulence analysis of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from Egyptian hospitals.Sci. Rep.2016613892910.1038/srep3892928004732
     [Google Scholar]
  31. Bialek-DavenetS. LavigneJ.P. GuyotK. MayerN. TournebizeR. BrisseS. Leflon-GuiboutV. Nicolas-ChanoineM.H. Differential contribution of AcrAB and OqxAB efflux pumps to multidrug resistance and virulence in Klebsiella pneumoniae.J. Antimicrob. Chemother.2015701818810.1093/jac/dku34025193085
     [Google Scholar]
  32. XuH. ZhouY. ZhaiX. DuZ. WuH. HanY. HuoC. ChenY. Emergence and characterization of tigecycline resistance in multidrug-resistant Klebsiella pneumoniae isolates from blood samples of patients in intensive care units in northern China.J. Med. Microbiol.201665875175910.1099/jmm.0.00029927324378
     [Google Scholar]
  33. FarivarA.S. NowrooziJ. EslamiG. SabokbarA. HashemiA. The study of antibiotic resistance among Klebsiella pneumoniae and expression level of oqxA and acrA genes by using real-time PCR.Resen. Med.2016404248
     [Google Scholar]
  34. CattoirV. PoirelL. RotimiV. SoussyC.J. NordmannP. Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates.J. Antimicrob. Chemother.200760239439710.1093/jac/dkm20417561500
     [Google Scholar]
  35. KhoshnoodS. HeidaryM. HashemiA. ShahiF. SakiM. KouhsariE. EslamiG. GoudarziH. Involvement of the AcrAB efflux pump in ciprofloxacin resistance in clinical Klebsiella pneumoniae isolates.Infect. Disord. Drug Targets202121456457110.2174/187152652099920090512122032888276
     [Google Scholar]
  36. VelebaM. De MajumdarS. HornseyM. WoodfordN. SchneidersT. Genetic characterization of tigecycline resistance in clinical isolates of Enterobacter cloacae and Enterobacter aerogenes.J. Antimicrob. Chemother.20136851011101810.1093/jac/dks53023349441
     [Google Scholar]
  37. PapstL. BeovićB. PulciniC. Durante-MangoniE. Rodríguez-BañoJ. KayeK.S. DaikosG.L. RakaL. PaulM. AbboL. AbgueguenP. AlmiranteB. AzziniA.M. Bani-SadrF. BassettiM. Ben-AmiR. BeovićB. BéraudG. Botelho-NeversE. BouG. BoutoilleD. CabiéA. CacopardoB. CascioA. CassirN. CastelliF. CecalaM. CharmillonA. ChirouzeC. CisnerosJ.M. ColmeneroJ.D. CoppolaN. CorcioneS. DaikosG.L. DallaG.D. De la CalleC.C. DelobelP. Di CaprioD. DuranteM.E. DuponM. EttaharN. FalagasM.E. FalconeM. FariñasM.C. FaureE. ForestierE. FotiG. GallagherJ. GattusoG. GendrinV. GentileI. GiacobbeD.R. GogosC.A. Grandiere PerezL. HansmannY. HorcajadaJ.P. IacobelloC. JacobJ.T. JustoJ.A. KernéisS. KomnosA. KotnikK.B. LebeauxD. Le BerreR. LechicheC. Le MoxingV. LescureF.X. LibanoreM. MartinotM. Merino deL.E. MondainV. MondelloP. MontejoM. MootienJ. MuñozP. Nir-PazR. PanA. Paño-PardoJ.R. PatelG. PaulM. Pérez RodríguezM.T. PirothL. PogueJ. PotoskiB.A. PourcherV. PyrpasopoulouA. RahavG. RizziM. Rodríguez-BañoJ. SalavertM. ScheetzM. SimsM. SpahijaG. StefaniS. StefosA. TammaP.D. TattevinP. TedescoA. Torre-CisnerosJ. TripolitsiotiP. TsiodrasS. UomoG. VerdonR. VialeP. VitratV. WeinbergerM. Wiener-WellY. Antibiotic treatment of infections caused by carbapenem-resistant Gram-negative bacilli: An international ESCMID cross-sectional survey among infectious diseases specialists practicing in large hospitals.Clin. Microbiol. Infect.201824101070107610.1016/j.cmi.2018.01.01529410094
     [Google Scholar]
  38. RuzinA. VisalliM.A. KeeneyD. BradfordP.A. Influence of transcriptional activator RamA on expression of multidrug efflux pump AcrAB and tigecycline susceptibility in Klebsiella pneumoniae.Antimicrob. Agents Chemother.20054931017102210.1128/AAC.49.3.1017‑1022.200515728897
     [Google Scholar]
  39. ChenY. HuD. ZhangQ. LiaoX.P. LiuY.H. SunJ. Efflux pump overexpression contributes to tigecycline heteroresistance in Salmonella enterica serovar Typhimurium.Front. Cell. Infect. Microbiol.201773710.3389/fcimb.2017.0003728261566
     [Google Scholar]
  40. ZhongX. XuH. ChenD. ZhouH. HuX. ChengG. First emergence of acrAB and oqxAB mediated tigecycline resistance in clinical isolates of Klebsiella pneumoniae pre-dating the use of tigecycline in a Chinese hospital.PLoS One2014912e11518510.1371/journal.pone.011518525503276
     [Google Scholar]
  41. JuanC.H. HuangY.W. LinY.T. YangT.C. WangF.D. Risk factors, outcomes, and mechanisms of tigecycline-nonsusceptible Klebsiella pneumoniae bacteremia.Antimicrob. Agents Chemother.201660127357736310.1128/AAC.01503‑1627697759
     [Google Scholar]
  42. ZhangC.Z. ChangM.X. YangL. LiuY.Y. ChenP.X. JiangH.X. Upregulation of AcrEF in quinolone resistance development in Escherichia coli when AcrAB-TolC function is impaired.Microb. Drug Resist.2018241182310.1089/mdr.2016.020728520511
     [Google Scholar]
/content/journals/cmp/10.2174/1874467217666230331081434
Loading
Antimicrobial Resistance of Clinical Klebsiella pneumoniae Isolates: Involvement of AcrAB and OqxAB Efflux Pumps
Curr Mol Pharmacol 17, E310323215266 (2024); https://doi.org/10.2174/1874467217666230331081434
/content/journals/cmp/10.2174/1874467217666230331081434
/content/journals/cmp/10.2174/1874467217666230331081434
Loading

Data & Media loading...


  • Article Type:     Research Article
Keyword(s): Antimicrobial; CCCP; Ciprofloxacin; Efflux pumps; Klebsiella pneumoniae; RT-PCR

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test