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2000
Volume 24, Issue 1
  • ISSN: 1566-5240
  • E-ISSN: 1875-5666

Abstract

Aim: We present a Direct SARS-CoV-2 Detection System that achieves sample-to-results in less than two hours in three simple steps.Methods: The Detection System includes Direct one-step Reverse Transcription PCR (RT-PCR) reagents (Qexp-MDx kit), a portable thermal cycler (Qampmini) with a preprogrammed chip and a simple-to-use Capillary Gel Electrophoresis system (Qsep Series Bio-Fragment Analyzer) with high fluorescence detection sensitivity to solve the problems associated with traditional real-time PCR (qPCR) systems which produces high false positive results.Results: The proposed simple-to-use detection platform can provide high detection sensitivity (identify less than 20 copies) and fast results (less than 120 minutes), which would be suitable for field testing applications.Conclusion: Our high detection sensitivity platform provides fast and accurate results in 120 minutes without doing DNA/RNA extraction.

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/content/journals/cmm/10.2174/1566524023666221104160148
2024-01-01
2025-07-06
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