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image of Anticancer and Cyclooxygenase Inhibitory Activity of Benzylidene Derivatives of Fenobam and its Thio Analogues

Abstract

Introduction

A series of benzylidene derivatives of fenobam and its thio analogues () have been evaluated for their cytotoxicity against breast cancer (MCF-7, MDA-MB-231), ovarian cancer (A2780, SKOV-3) and cervical cancer (HELA) cell lines.

Method

These compounds () exhibited 72-83% inhibition of Erk activity against the ovarian cancer cell line (A2780). Compounds and showed the highest DNA damage effect in Comet Assay against the A2780 cancer cell line as compared to the other tested analogues ( and ) by using % Tail DNA and OTM. Compounds , and 11 showed significant activities and selectivity towards COX-2 with 78%, 97%, and 89% inhibition, as compared to 17%, 57%, and 26% inhibition against COX-1 isoenzyme, respectively.

Results

Interestingly, molecular docking scores were also in very good agreement with the experimental results regarding discriminating the selectivity index of the tested compounds against COX-1 & COX-2 enzymes. Further molecular dynamics (MD) simulation study revealed that the most selective compound, 13, binds with the COX-2 enzyme in a similar fashion to that of Rofecoxib, which was further supported by their MD-based free binding energies (MM-GBSA) of -49.76 ± 4.27 kcal/mol, and -44.84 ±3.78 kcal/mol, respectively.

Conclusion

Moreover, ADMET predictions showed adequate properties for these compounds, making them promising leads worthy of further optimization.

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2024-12-16
2025-01-18

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/content/journals/cmc/10.2174/0109298673345068241120045638
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Anticancer and Cyclooxygenase Inhibitory Activity of Benzylidene Derivatives of Fenobam and its Thio Analogues
Bentham Science Publishers ; https://doi.org/10.2174/0109298673345068241120045638
/content/journals/cmc/10.2174/0109298673345068241120045638
/content/journals/cmc/10.2174/0109298673345068241120045638
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  • Article Type:     Research Article
Keywords: Erk analysis ; COX 1 inhibition ; Benzylidene derivatives ; COMET assay ; COX 2 inhibition ; cancer

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