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image of Elucidating the Mechanisms of Astragalus Membranaceus in Colorectal Cancer Patients through Bioinformatics Analysis

Abstract

Background

Astragalus membranaceus has shown positive clinical efficacy in treating colorectal cancer (CRC).

Objective

This study aimed to identify the key active components of Astragalus and determine effective targets of these components in CRC patients.

Methods

We identified active components of Astragalus membranaceus and differentially expressed genes in traditional Chinese medicine systems pharmacology database and The Cancer Genome Atlas. Additionally, the enrichment analysis of differential target genes (DTGs) was performed using the R-package clusterProfiler. Immunocyte correlation analysis and non-coding regulatory network construction were performed for biomarkers using Spearman’s method and NetworkAnalyst. Finally, molecular docking of biomarkers and their corresponding molecule drugs was done with Autodock Vina software.

Results

We identified 20 active components of Astragalus membranaceus and 1 403 target genes through screening. A total of 2 300 differentially expressed genes, and 3 035 hub genes in CRC were screened. The integration of the target genes with the significantly differentially expressed genes and Hub genes identified resulted in a total of 86 DTGs. Subsequently, the results showed 828 enriched GO biological processes, 184 enriched GO molecular functions, 59 enriched GO cellular components, and 46 enriched KEGG pathways. We also obtained a total of 143 PPI pairs involving 67 nodes. Additionally, we constructed 45 mRNA-TF pairs, 101 miRNA-mRNA pairs, and 200 miRNA-mRNA-TF triplets. Finally, molecular docking was performed for the active component quercetin with F2 and UGT1A1 and formic acid with FGA, AHSG, and KNG1.

Conclusion

This study identified the active components of Astragalus membranaceus and their corresponding targets in CRC. These findings provide robust evidence for precision drug therapy in patients with CRC.

© 2024 Bentham Science Publishers
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2024-10-31
2024-11-26

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Elucidating the Mechanisms of Astragalus Membranaceus in Colorectal Cancer Patients through Bioinformatics Analysis
Bentham Science Publishers ; https://doi.org/10.2174/0109298673344265241014114804
/content/journals/cmc/10.2174/0109298673344265241014114804
/content/journals/cmc/10.2174/0109298673344265241014114804
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  • Article Type:     Research Article
Keywords: target gene ; Colorectal cancer ; active component ; astragalus membranaceus ; bioinformatics analysis ; drug therapy

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