Current Metabolomics - Volume 3, Issue 1, 2015

The importance of nucleoside metabolism in brain followed the recognition that i) adult nervous system maintains its nucleotide pools in the proper qualitative and quantitative balance by salvaging preformed purine and pyrimidine rings, rather than by synthesizing nucleosides de novo from simple precursors, ii) adenosine, a purine nucleoside, acts as an extracellular signal, and exerts its protective effects by interacting with Read More

While data processing methods in metabolomic studies often work with 'n' number of dimensions, analytical techniques, with the notable exception of NMR, have mostly stuck only to one. Peak overlap continues to be a problem and there is an ever-present demand to maximize the number of metabolites that can be separated and identified in a single run. One method that might help to overcome these issues is multidimensio Read More

In high-throughput scenarios, a large number of similar NMR spectra from a single study need to be analyzed. The complexity of the data required the use of spectral databases to characterize resonances of interest and extract quantitative information. The recently reported CRAFT (Complete Reduction to Amplitude Frequency Table) technique, based on a Bayesian analysis approach, converts a time-domain FID to a frequency Read More

Polar metabolite profiles (amino acids, organic acids, phenols, sugars and sugar alcohols) and nonpolar metabolites (mainly fatty acids) of 11 different varieties of Onion (Allium cepa L.) bulb developed in India, were analyzed using GC-MS based metabolomics approach. PLS-DA score plot was used for visual assessments of clustering trends. The metabolites contributing for formation of segregated clustering scores were id Read More

Nine samples of propolis from different parts of the UK were extracted and profiled by high resolution LC-MS. The data were aligned and features were extracted into 0.02 amu windows. The profiles contained thousands of features. In order to establish a platform for comparison of the samples the top 125 features by average peak intensity across the samples, after excluding abundant dimer peaks, were selected for fu Read More

The field of metabolomics has continued to expand rapidly in the past two years. It has become an important part of personalized, predictive, preventive, and participatory (P4) medicine. In this article, recent advances in analytical methods (capillary electrophoresis, gas chromatography, liquid chromatography, mass spectrometry and MS imaging) are described. Advances in data analysis, quality control and receiver oper Read More

Infection by the Human Immunodeficiency Virus (HIV) and subsequent treatment with Highly Active Antiretroviral therapy (HAART) can lead to the development of metabolic abnormalities. The ability to diagnose and monitor these complications remains cumbersome and is limited to the detection of one or only a few metabolites at a time. The discovery and development of reliable HIV and/or HAART related biomarkers, u Read More