Combinatorial Chemistry & High Throughput Screening - Volume 13, Issue 1, 2010

After serving as Editor-in-Chief of Combinatorial Chemistry & High Throughput Screening since the beginning of its publication in 1998 and overseeing the first 12 volumes, I am stepping down from this position with the beginning of CCHTS volume 13. Since I will remain on the Editorial Board, I will still be involved with CCHTS. The new Editor-in-Chief of CCHTS will be Dr. Rathnam Chaguturu who directs the High Through Read More

Stimulation of CXC-type chemokine receptor 2 (CXCR2)-transfected cells by Gro-α or IL-8 induced (i) CXCR2 internalization, (ii) phosphorylation of ERK1/2 (pERK) and (iii) translocation of nuclear factor of activated T-cells (NFAT) into the nucleus. Employing high content screening (HCS; i.e. fluorimetric imaging combined with image analysis) these three ligand-induced events were quantified by using a CXCR2-specific antibo Read More

A dynamic, focused screening strategy that utilized a limited but diversified set of target-specific compounds was explored as an efficient means for the identification of inhibitors of the protein kinase PDK1. Approximately 21,500 compounds, including a 19,000 molecule kinase-focused compound collection (KFCC), were screened at two concentrations to identify initial leads. The KFCC included several empirically-derived, general ki Read More

The malachite green assay is often used for measuring the presence of inorganic mono-phosphate concentrations. Some studies have adapted this assay for use in monitoring enzymatic reactions and have suggested its potential use in high throughput screening (HTS). With the increasing availability of laboratory automation, some studies are starting to explore the possibility of conducting limited, semi-automated versions Read More

A quantitative structure &ndash property relationship (QSPR) was used to predict the critical micelle concentration (cmc) of nonionic surfactants. The relation was developed for a diverse set of 23 nonionic surfactants (r = 0.99, F-test = 1042.5) employing the connectivity and valence connectivity indices only. The molecular connectivity indices were calculated for the whole molecule which was a simple and general approach.

With the increasingly available technology in automated synthesis and screening protocols, the combination of polymer-supported chemistry and biocatalysis, with their respective advantages over classical organic synthesis, has become more scientifically attractive, yet remains challenging. Since the development of solid-phase synthesis and its rapid expansion in combination with the advent of combinatorial techniques, a va Read More

Nature, especially the plant kingdom, is a rich source for novel bioactive compounds that can be used as lead compounds for drug development. In order to exploit this resource, the two neural network-based virtual screening techniques novelty detection with self-organizing maps (SOMs) and counterpropagation neural network were evaluated as tools for efficient lead structure discovery. As application scenario, significant des Read More

Nanoparticles of 35 individual metals as well as their binary combinations were synthesized using High Throughput pulsed laser ablation (PLA), and collected on Al2O3, CeO2, SiO2, TiO2, and ZrO2 pellets. These materials were then screened for their catalytic activities and selectivities for the partial oxidation of propylene, in particular for propylene oxide (PO), using array channel microreactors. Reaction conditions were the fo Read More

Phage-display selection of combinatorial libraries is a powerful technique for identifying binding ligands against desired targets. Evaluation of target binding capacity of multiple clones recovered from phage display selection to a specific target is laborious, time-consuming, and a rate-limiting step. We constructed phage-display combinatorial peptide libraries in fusion with a β-lactamase enzyme, which acts as a reporter. Linea Read More