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Volume 27, Issue 18
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Background

Gastric cancer, one of the most familiar adenocarcinomas of the gastrointestinal tract, ranks third in the world in cancer-related deaths. Traditional Chinese medicine can suppress the growth of tumors, and the underlying mechanism may be associated with the tumor microenvironment. Here, we investigated the anti-cancer effects of the on gastric cancer and the underlying molecular mechanism.

Methods

An nude mouse model was established, and the expression of CD206, CD80, and M2 phenotype-related proteins (Arg-1, Fizz1) was obtained by flow cytometry and western blotting. The expressions of the M2 phenotype-related cytokines were examined by ELISA.

Results

inhibited gastric tumor growth and downregulated the expression of CD206, IFN-γ, IL-13, IL-4, and TNF-α . deceased M2 phenotypic polarization by upregulating microRNA (miR)-29a-3p level. Luciferase activity assays showed that HDAC4 is a potential target of miR-29a-3p. In cells co-transfected with HDAC4 siRNA and miR-29a-3p inhibitor and treated with IL-4 and , the miR-29a-3p inhibitor-induced increase of M2 phenotypic polarization was reversed.

Conclusion

In summary, these results suggested that the regulated M2 macrophage polarization by regulating miR-29a-3p/HDAC4, providing a different and innovative treatment for gastric cancer.

© 2024 Bentham Science Publishers
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2023-10-16
2025-01-13

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Qingrexiaoji Recipe Regulates the Differentiation of M2 TAM via miR-29 in GC
Comb Chem High Throughput Screen 27, 2764 (2024); https://doi.org/10.2174/0113862073263776231009115524
/content/journals/cchts/10.2174/0113862073263776231009115524
/content/journals/cchts/10.2174/0113862073263776231009115524
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  • Article Type:     Research Article
Keyword(s): gastric cancer; HDAC4; M2; miR-29; Qingrexiaoji recipe; tumor-associated macrophages (TAMs)

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