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Volume 27, Issue 18
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Introduction

Ras-related protein in brain 4A (Rab4A), as a member of the Rab family, is involved in the intracellular circulation of membrane receptors or endocytic substances and regulates the progression of multiple tumors.

Methods

From our results, the knockdown of Rab4A inhibited the proliferation, migration and invasion in AGS cells. Importantly, the surface expression of epidermal growth factor receptor (EGFR) declined significantly in Rab4A knockdown cells. The downstream pathway of EGFR was also inhibited after the transfection of Rab4A-specific siRNA, including AKT and β-catenin pathways.

Results

In addition, miR-496 down-regulated the expression of Rab4A in AGS cells. The result of the luciferase reporter assay showed that miR-496 could bind to the 3’UTR of Rab4A.

Conclusion

In conclusion, the expression of Rab4A is inhibited by miR-496, and the knockdown of Rab4A inhibits the proliferation, migration and invasion through down-regulating the surface expression of EGFR. Rab4A is a potential target in the treatment of gastric cancer.

© 2024 Bentham Science Publishers
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2023-10-16
2025-01-13

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Ras-related Protein in Brain 4A (Rab4A) is Downregulated by miR-496 to inhibit the Progression of Gastric Cancer
Comb Chem High Throughput Screen 27, 2734 (2024); https://doi.org/10.2174/0113862073260841231010055245
/content/journals/cchts/10.2174/0113862073260841231010055245
/content/journals/cchts/10.2174/0113862073260841231010055245
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  • Article Type:     Research Article
Keyword(s): Epidermal growth factor receptor; gastric cancer; invasion in AGS cells; miRNA; ras-related protein in brain 4A (Rab4A); surface expression

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