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Volume 27, Issue 18
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Background

Multiple brain disorders are treated by Scutellaria Radix (SR), including cerebral ischemia-reperfusion (CI/R). However, more studies are needed to clarify the molecular mechanism of SR for CI/R.

Methods

The active substances and potential targets of SR and CI/R-related genes were obtained through public databases. Overlapping targets of SR and CI/R were analyzed using protein-protein interaction (PPI) networks. GO and KEGG enrichment analyses were performed to predict the pathways of SR against CI/R, and the key components and targets were screened for molecular docking. The results of network pharmacology analysis were verified using experiments.

Results

15 components and 64 overlapping targets related to SR and CI/R were obtained. The top targets were AKT1, IL-6, CAS3, TNF, and TP53. These targets have been studied by GO and KEGG to be connected to a number of signaling pathways, including MAPK, PI3K-Akt pathway, and apoptosis. Molecular docking and cell experiments helped to further substantiate the network pharmacology results.

Conclusion

The active compound of SR was able to significantly decrease the apoptosis of HT-22 cells induced by OGD/R. This finding suggests that SR is a potentially effective treatment for CI/R by modulating the MAPK and PI3K-Akt pathways.

© 2024 Bentham Science Publishers
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2023-10-19
2025-01-13

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Network Pharmacology and Molecular Docking Analysis on Mechanisms of Scutellariae Radix in the Treatment of Cerebral Ischemia-reperfusion Injury
Comb Chem High Throughput Screen 27, 2712 (2024); https://doi.org/10.2174/0113862073258863230921180641
/content/journals/cchts/10.2174/0113862073258863230921180641
/content/journals/cchts/10.2174/0113862073258863230921180641
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  • Article Type:     Research Article
Keyword(s): apoptosis; baicalin; cerebral ischemia/reperfusion; MAPK signaling pathway; molecular docking; Network pharmacology; PI3K/AKT signaling pathway; Scutellariae Radix

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