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Volume 28, Issue 1
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Background

Asthma is a chronic inflammatory disease of the airways that seriously endangers human health. Belamcanda chinensis (BC), a traditional Chinese medicine, has been used to counteract asthma as it has been shown to possess anti-inflammatory and regulatory immunity properties.

Objective

The study aimed to investigate the mechanisms of action of BC in the treatment of asthma; a “dose–effect weighted coefficient” network pharmacology method was established to predict potential active compounds.

Methods

Information on the components and content of BC was obtained by UPLC-QE-Orbitrap-MS spectrometry. Based on BC content, oral bioavailability, and molecular docking binding energy, dose-effect weighting coefficients were constructed. With the degree greater than average as the index, a protein–protein interaction (PPI) database was used to obtain the core key targets for asthma under dose–effect weighting. GO function and KEGG pathway analyses of the core targets were performed using DAVID software. Finally, MTT and ELISA assays were used to assess the effects of active components on 16HBE cell proliferation.

Results

The experimental results using the 16HBE model demonstrated BC to have a potential protective effect on asthma. Network pharmacology showed SYK, AKT1, and ALOX5 to be the main key targets, and Fc epsilon RI as the promising signaling pathway. Eight components, such as tectoridin, mangiferin, luteolin, and isovitexin were the main active compounds, Finally, we analyzed the LPS-induced 16HBE proliferation of each active ingredient. Based on the activity verification study, all five predicted components promoted the proliferation of 16HBE cells. These five compounds can be used as potential quality markers for asthma.

Conclusion

This study provides a virtual and practical method for the simple and rapid screening of active ingredients in natural products.

© 2025 Bentham Science Publishers
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2023-11-22
2025-01-19

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References

  1. WoźniakD. MatkowskiA. Belamcandae chinensis rhizoma – a review of phytochemistry and bioactivity.Fitoterapia201510711410.1016/j.fitote.2015.08.015 26347953
     [Google Scholar]
  2. YeQ. ZhangQ. YaoH. XuA. LiuY. QiJ. ZhangH. ZhangJ. Active-Ingredient Screening and Synergistic Action Mechanism of Shegan Mixture for Anti-Asthma Effects Based on Network Pharmacology in a Mouse Model of Asthma.Drug Des. Devel. Ther.2021151765177710.2147/DDDT.S288829 33953545
     [Google Scholar]
  3. LinC.C. WangY.Y. ChenS.M. LiuY.T. LiJ.Q. LiF. DaiJ.C. ZhangT. QiuF. LiuH. DaiZ. ZhangZ.D. Shegan-Mahuang Decoction ameliorates asthmatic airway hyperresponsiveness by downregulating Th2/Th17 cells but upregulating CD4+FoxP3+ Tregs.J. Ethnopharmacol.202025311265610.1016/j.jep.2020.112656 32035217
     [Google Scholar]
  4. PorsbjergC. MelénE. LehtimäkiL. ShawD. Asthma.Lancet20234011037985887310.1016/S0140‑6736(22)02125‑0 36682372
     [Google Scholar]
  5. SelbergS. HedmanL. JanssonS.A. BackmanH. StridsmanC. Asthma control and acute healthcare visits among young adults with asthma—A population‐based study.J. Adv. Nurs.201975123525353410.1111/jan.14174 31441107
     [Google Scholar]
  6. LommatzschM. BrusselleG.G. CanonicaG.W. JacksonD.J. NairP. BuhlR. VirchowJ.C. Disease-modifying anti-asthmatic drugs.Lancet2022399103351664166810.1016/S0140‑6736(22)00331‑2 35461560
     [Google Scholar]
  7. LeeJ.W. LeeC. JinQ. LeeM.S. KimY. HongJ.T. LeeM.K. HwangB.Y. Chemical constituents from Belamcanda chinensis and their inhibitory effects on nitric oxide production in RAW 264.7 macrophage cells.Arch. Pharm. Res.201538699199710.1007/s12272‑014‑0529‑8 25502561
     [Google Scholar]
  8. SzandrukM. Merwid-LądA. SzelągA. The impact of mangiferin from Belamcanda chinensis on experimental colitis in rats.Inflammopharmacology201826257158110.1007/s10787‑017‑0337‑0 28337639
     [Google Scholar]
  9. ZhouZ. ChenB. ChenS. LinM. ChenY. JinS. ChenW. ZhangY. Applications of Network Pharmacology in Traditional Chinese Medicine Research.Evid. Based Complement. Alternat. Med.202020201710.1155/2020/1646905 32148533
     [Google Scholar]
  10. ShiS. JiX. ShiJ. ShiS. SheF. ZhangQ. DongY. CuiH. HuY. Andrographolide in atherosclerosis: integrating network pharmacology and in vitro pharmacological evaluation.Biosci. Rep.2022427BSR2021281210.1042/BSR20212812 35543243
     [Google Scholar]
  11. WangY. ZouJ. JiaY. ZhangX. WangC. ShiY. GuoD. WuZ. WangF. The Mechanism of Lavender Essential Oil in the Treatment of Acute Colitis Based on “Quantity–Effect” Weight Coefficient Network Pharmacology.Front. Pharmacol.20211264414010.3389/fphar.2021.644140 33981227
     [Google Scholar]
  12. MaH. FuW. YuH. XuY. XiaoL. ZhangY. WuY. LiuX. ChenY. XuT. Exploration of the anti-inflammatory mechanism of Lanqin oral solution based on the network pharmacology analysis optimized by Q-markers selection.Comput. Biol. Med.202315410660710.1016/j.compbiomed.2023.106607 36731363
     [Google Scholar]
  13. GaoX.C. ZhangN.X. ShenJ.M. LvJ.W. ZhangK.Y. SunY. LiH. WangY.L. ChengD.D. ZhaoM.Y. ZhangH. LiC.N. SunJ.M. Screening of the Active Compounds against Neural Oxidative Damage from Ginseng Phloem Using UPLC-Q-Exactive-MS/MS Coupled with the Content-Effect Weighted Method.Molecules20222724906110.3390/molecules27249061 36558193
     [Google Scholar]
  14. YangK.M. ChaoL.K. WuC.S. YeZ.S. ChenH.C. Headspace Solid-Phase Microextraction Analysis of Volatile Components in Peanut Oil.Molecules20212611330610.3390/molecules26113306 34072807
     [Google Scholar]
  15. GaoK. SongY.P. SongA. Exploring active ingredients and function mechanisms of Ephedra-bitter almond for prevention and treatment of Corona virus disease 2019 (COVID-19) based on network pharmacology.BioData Min.20201311910.1186/s13040‑020‑00229‑4 33292385
     [Google Scholar]
  16. JiangN. LiH. SunY. ZengJ. YangF. KantawongF. WuJ. Network Pharmacology and Pharmacological Evaluation Reveals the Mechanism of the Sanguisorba Officinalis in Suppressing Hepatocellular Carcinoma.Front. Pharmacol.20211261852210.3389/fphar.2021.618522 33746755
     [Google Scholar]
  17. LiuX. XiangD. JinW. ZhaoG. LiH. XieB. GuX. Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro.Bioengineered20221323450346110.1080/21655979.2021.2024685 35094658
     [Google Scholar]
  18. CorryD.B. RishiK. KanellisJ. KissA. SongL. XuJ. FengL. WerbZ. KheradmandF. Decreased allergic lung inflammatory cell egression and increased susceptibility to asphyxiation in MMP2-deficiency.Nat. Immunol.20023434735310.1038/ni773 11887181
     [Google Scholar]
  19. VermeerP.D. DenkerJ. EstinM. MoningerT.O. KeshavjeeS. KarpP. KlineJ.N. ZabnerJ. MMP9 modulates tight junction integrity and cell viability in human airway epithelia.Am. J. Physiol. Lung Cell. Mol. Physiol.20092965L751L76210.1152/ajplung.90578.2008 19270179
     [Google Scholar]
  20. VillaltaP.C. RocicP. TownsleyM.I. Role of MMP2 and MMP9 in TRPV4-induced lung injury.Am. J. Physiol. Lung Cell. Mol. Physiol.20143078L652L65910.1152/ajplung.00113.2014 25150065
     [Google Scholar]
  21. LeeS.J. ChoiJ.S. BongS.M. HwangH.J. LeeJ. SongH.J. LeeJ. KimJ.H. KohJ.S. LeeB.I. Crystal Structures of Spleen Tyrosine Kinase in Complex with Two Novel 4-Aminopyrido[4,3-d] Pyrimidine Derivative Inhibitors.Mol. Cells201841654555210.1007/s10059‑010‑0161‑5 29890824
     [Google Scholar]
  22. CrozierR.W.E. YousefM. CoishJ.M. FajardoV.A. TsianiE. MacNeilA.J. Carnosic acid inhibits secretion of allergic inflammatory mediators in IgE-activated mast cells via direct regulation of Syk activation.J. Biol. Chem.2023299410286710.1016/j.jbc.2022.102867 36608933
     [Google Scholar]
  23. ZhaoT.X. MallatZ. Targeting the Immune System in Atherosclerosis.J. Am. Coll. Cardiol.201973131691170610.1016/j.jacc.2018.12.083 30947923
     [Google Scholar]
  24. García-MenayaJ.M. Cordobés-DuránC. García-MartínE. AgúndezJ.A.G. Pharmacogenetic Factors Affecting Asthma Treatment Response. Potential Implications for Drug Therapy.Front. Pharmacol.20191052010.3389/fphar.2019.00520 31178722
     [Google Scholar]
  25. ChanezP. Contin-BordesC. GarciaG. VerkindreC. DidierA. De BlayF. Tunon de LaraM. BlancoP. MoreauJ.F. RobinsonP. BourdeixI. TrunetP. Le GrosV. HumbertM. MolimardM. Omalizumab-induced decrease of FcɛRI expression in patients with severe allergic asthma.Respir. Med.2010104111608161710.1016/j.rmed.2010.07.011 20801010
     [Google Scholar]
  26. ClyneA. YangL. YangM. MayB. YangA.W.H. Molecular docking and network connections of active compounds from the classical herbal formula Ding Chuan Tang.PeerJ20208e868510.7717/peerj.8685 32185106
     [Google Scholar]
  27. GuoH.W. YunC.X. HouG.H. DuJ. HuangX. LuY. KellerE.T. ZhangJ. DengJ.G. Mangiferin attenuates TH1/TH2 cytokine imbalance in an ovalbumin-induced asthmatic mouse model.PLoS One201496e10039410.1371/journal.pone.0100394 24955743
     [Google Scholar]
  28. WangS. WuniqiemuT. TangW. TengF. BianQ. YiL. QinJ. ZhuX. WeiY. DongJ. Luteolin inhibits autophagy in allergic asthma by activating PI3K/Akt/mTOR signaling and inhibiting Beclin-1-PI3KC3 complex.Int. Immunopharmacol.20219410746010.1016/j.intimp.2021.107460 33621850
     [Google Scholar]
  29. QiaoX. FengT. ZhangD. ZhiL. ZhangJ. LiuX. PanY. XuJ. CuiW.J. DongL. Luteolin alleviated neutrophilic asthma by inhibiting IL-36γ secretion-mediated MAPK pathways.Pharm. Biol.202361116517610.1080/13880209.2022.2160770 36604842
     [Google Scholar]
  30. LiJ. ZhangB. Apigenin protects ovalbumin-induced asthma through the regulation of Th17 cells.Fitoterapia20139129830410.1016/j.fitote.2013.09.009 24060907
     [Google Scholar]
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Exploring Asthma Mechanism of Belamcanda Chinensis by “Dose–effect Weighted Coefficient” Network Pharmacology and Experiment Validation
Comb Chem High Throughput Screen 28, 33 (2025); https://doi.org/10.2174/0113862073254607231107074015
/content/journals/cchts/10.2174/0113862073254607231107074015
/content/journals/cchts/10.2174/0113862073254607231107074015
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  • Article Type:     Research Article
Keyword(s): asthma; Belamcanda chinensis; protein–protein interaction (PPI); quality markers for asthma; UPLC-QE-Orbitrap-MS

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