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image of Chemical Profiling, In-Silico Investigation and In Vivo Toxicity Assessment of Lacatomtom (A Psychoactive Mixture) on Selected Indices in Albino Wistar Rats

Abstract

Introduction/Objective

The use of lacatomtom (LTT), a psychoactive mixture of tomtom (TT) candies with lacasera (LC) beverage, has recently increased among young Nigerians and Africans. There isn't much scientific study on the constituent and effects of this psychoactive substance.

Methods

Herein, LTT was chemically-profiled using GCMS analysis, and the toxicological effects were examined in albino rats. experiment consists of five groups of six rats each (group 2 - LTT ; groups 1, 3, & 4 - TTT, TT, LC (1 mL) mg/mL kg/body weight once/day respectively, group 5 - distilled water ). Identified constituents were examined against human monoamine oxidase (MOA) and human catechol O-methyltransferase (COMT) using methods.

Results

Forty-seven chemical compounds were identified. intake of LTT elevated plasma alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatinine, total cholesterol, and LDL-cholesterol levels. The docked poses, binding scores, and interactions with amino acids informed the selection of (4-Methoxymethoxy-hex-5-ynylidene)-cyclohexane (MM) (-9.4 kcal/mol) and 3-(hydroxyphenylmethyl)-3,4-dimethyl-1-phenylpentan-2-one (HP) for COMT (-9.4 kcal/mol), while propionylcodeine (-10.1 kcal/mol) and HP (-8.9 kcal/mol) for MOA. Top-docked compounds (TDC) demonstrated the potential to permeate the blood-brain barrier. TDC was predicted to be a positive substrate of the P-glycoprotein and presents inhibitory potential for cytochrome P descriptors. HP was mutagenic and could induce human hepatotoxicity and drug-induced liver injury, while propionylcodeine had a human hepatotoxic prediction.

Conclusion

The present study, for the first time, confirmed the potential toxicity of lacatomtom to the liver, kidney, heart, and central nervous system supported by the identified top-docked compounds regarded as potential psychoactive constituents of MOA and COMT.

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2024-11-04
2024-12-23

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/content/journals/ccb/10.2174/0122127968319253241024050426
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Chemical Profiling, In-Silico Investigation and In Vivo Toxicity Assessment of Lacatomtom (A Psychoactive Mixture) on Selected Indices in Albino Wistar Rats
Bentham Science Publishers ; https://doi.org/10.2174/0122127968319253241024050426
/content/journals/ccb/10.2174/0122127968319253241024050426
/content/journals/ccb/10.2174/0122127968319253241024050426
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  • Article Type:     Research Article
Keywords: Lacatomtom ; monoamine oxidase ; ADMET analysis ; liver function test ; catechol O-methyltransferase ; molecular docking

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