Current Biotechnology - Volume 4, Issue 1, 2015

This paper aims to introduce an efficient predictive function for total energy expenditure (TEE) in everyday life using dedicated mass-market sensors similar to those found in widespread smartphones and tablets. Our research encompasses the design of a TEE estimation model using the smartphone accelerometer with a new signal-to-energy transformation function. The main idea of this study consists in using the signal i Read More

In the multiple sequence alignment (MSA) of the members of a protein family some positions are highly conserved, while others vary. The conserved positions are clearly important, but the non-conserved positions are also important because the destabilizing effects of a given amino acid at one position can be compensated by the stabilizing effect of a certain amino acid at another position: in other words, two (or mo Read More

Transcription factors control the flow of genetic information from DNA to messenger RNA rendering understanding of their function highly important for the modulation and optimization of protein expression. Molecular and structural biology have provided a wealth of information for transcription factors, however, the dynamic processes underlying their activation are yet not fully understood. In this context, molecular Read More

The rapid development of mass spectrometry-based proteomic technologies has allowed the quantification and validation of protein biomarkers toward detection of signature molecules, called proteotypic peptides. To facilitate their extraction from experimental protein and peptide lists, we present here a friendly computational tool called Experimental Proteotypic Peptides Investigator (EPPI). In this study, it was used for extr Read More

With the advancement in proteomics and bioinformatics, it is paramount to predict the causes of aggregation in all Human Hydrolase Enzymes (HHE), which have more tendencies to aggregate. Protein aggregation is associated with manifold pathological and neurodegenerative diseases because of amyloid fibrillation. Physico-chemical factors responsible for aggregation were studied in details. The positional dependencie Read More

Stevioside, a glycoside present in the leaves of Stevia rebaudiana Bertoni, offers therapeutic benefits such as anti-hyperglycemic, anti-hypertensive, antiinflammatory, anti-tumor, diuretic and immune influencing properties. In this work antimicrobial activity of stevioside against Bacillus cereus, a major source of milk contamination was investigated. The isolate was confirmed by various biochemical and 16S rRNA Read More

Novel short-chain-length-long-chain-length polyhydroxyalkanoate (SCL-LCL-PHA) copolymer production was examined with Pseudomonas aeruginosa MTCC 7925 under supplementation of non-edible oils such as karanja, jatropha, mahua, and castor oils, and their respective cakes for cost reduction. Polymer yield reached up to 4.66 g/l (63.7% dry cell wt., dcw) with a mol fraction of 89.7:4.2:2.7:3.4 of 3- hydroxybutyric Read More