Current Biotechnology - Volume 3, Issue 4, 2014

The development of cancer is associated with alterations to the physiology of the cell caused by multiple changes in its genome. A frequent genomic event in cancer is chromosome translocation, an exchange of large DNA fragments between two non-homologous chromosomes that in many cases leads to the creation of new fusion genes. Chromosome translocations are key events in the initiation or progression of man Read More

Leishmaniasis is defined as a cluster of infectious diseases caused by protozoan parasites of the genus Leishmania. It is recognized worldwide as a major health problem, showing high endemicity in developing countries. The present review details this complex group of neglected diseases, with important clinical spectrum and large epidemiological diversity, and discusses the evolution of leishmaniasis treatment and the urgent n Read More

The aim of the study was to compare the optimum immobilization conditions of lipase obtained from thermophilic Bacillus aerius and commercial lipase ‘Lipolase’. Both bacterial and commercial lipase were immobilized by surface adsorption onto silica matrix (100-200μm mesh with pore size 2.4 nm) and subsequently exposed to 4% glutaraldehyde, showed binding efficiency of 96.25% and 75.64%, respectively. Maximum enzy Read More

An extracellular cholesterol oxidase (COX) from Bacillus sp. COX-T3 was successfully purified from the cell free culture broth by successive steps of ammonium sulphate precipitation, dialysis and Riboflavin-affinity column chromatography to ∼28.4-fold with an over all yield of 6.2%. The purified COX showed a single band of Mr 21 kDa in SDS-PAGE and ~99 kDa band in Native- PAGE thus indicating an oligomeric nature of the enzy Read More

Cyclodextrin glycosyltransferase (CGTase) from the thermophilic anaerobe Thermoanaerobacter sp. 5K was purified and characterized. The enzyme was purified with ammonium sulfate precipitation followed by α-CD-bound, epoxy-activated Sepharose 6B affinity chromatography. Molecular weight of the purified enzyme was 70.6 kDa. The enzyme had optimal activity at 80-90°C and retained greater than 90% activ Read More

Bulk production of recombinant proteins and therapeutic vaccines largely depends on cellculture techniques. Suspension culture using microcarrier is a common manufacturing practice, despite its production limitations caused by shear force damage. Here we report a simple and scalable method to overcome this and improve the healthy cell mass. We hypothesized to curb the cell damage and increase the yield of cellular Read More

The biotechnological production of intracellular tyrosine phenol lyase finds important applications in pharmaceutical industry for the development of novel therapeutic molecules like L-DOPA. Cell disruption is one of the important steps in the downstream processing of intracellular enzymes and bioprocess industry demands this operation to be energy, cost and time efficient. The efficiency of the disruption process for the release Read More