Current Biotechnology - Volume 3, Issue 2, 2014

To overcome food borne illness, food quality and safety are major scientific disciplines including food handling, preparation and storage. Detection of contaminants in the food system is the main concern regarding public health. It is necessary to develop an immunoassay system to detect and control food borne pathogens in contaminated foods before they enter into the human food chain and cause infections. The convention Read More

Improving the performance of their bioreactors is a continuous distinguished task of process engineers for the entire lifetime of their processes. In this paper new developments which help to improve the performance of bioreactors for recombinant protein formation are reviewed. In order to judge the performance of a cultivation process an important prerequisite is accurately and easily monitoring the key quantities of their p Read More

A bacterial strain producing extracellular alkaline thermostable laccase has been isolated from sawdust effluents and identified as Micrococcus species. The laccase was partially purified and characterized. The isolate showed maximum laccase production after 120 h, at 30ºC with an optimum pH of 9.0. The specific activity was 127.94 U/mg with a purification fold of 5.54 was obtained with DEAE-cellulose anion exchan Read More

A purified thermo-tolerant lipase of Streptomyces STL-D8 was immobilized onto the natural fibres namely ‘Bombax’. Hydrolytic activity of this fibre-bound lipase was determined for hydrolysis of p-NPP in a 0.05 M Tris buffer (pH 8.5) at 55°C. This fibre-bound lipase was used to synthesize methyl succinate using methanol and succinic acid as reactants in DMSO at 55°C under shaking. Although a low yield of methyl succinate was o Read More

The effects of selected yeast extract (YE) compounds on 2,3-butanediol production by Paenibacillus polymyxa DSM 365 were studied. The intention was to substitute complex sources of nutrients by defined compounds thus reducing the high demand of YE as well as lowering the costs of fermentation media. For this purpose the depletion of different inorganic compounds during cultivations was analyzed. Then, their impac Read More

The problem of uneven and faulty dyeing in the finished quality of fabrics is usually tackled through a chemical stripping process to remove the loose dyes from the surface of dyed cotton fabrics. This research work was undertaken to investigate the color stripping of cotton fabric dyed with C.I. reactive black 5 in an environmentally-friendly way using enzymes instead of applying chemicals. Such bio-degradable and economi Read More

The aim of this study was to evaluate the utility of isolated fungus in biodegradation of non-sterile molasses spent wash (MSW). The non-sterile MSW was used to check the biodegradation capabilities of isolated fungus in the presence of other contaminants. The irrigation site for culture isolation was selected to verify the presence of microbes that are suitable for MSW biodegradation. Three different fungi were isolated f Read More

To facilitate the use of biotechnological techniques for conservation, germplasm exchange and improvement of Tetrapleura tetraptera, the study investigated in vitro plant regeneration via somatic embryogenesis from isolated axillary meristem of the plant. Indirect primary somatic embryos were induced on MS basal medium supplemented with nine different concentrations of 2,4-D. Pre-embryogenic calli were made to matu Read More

Endo-glucanase (CMCase) is widely used in detergents, textile and biofuel industry as color caring agent, smooth surfacing agent of cotton fabrics and biofuels production from plant biomass. Extracellular CMCase of Scytalidium thermophilum grown on 2.0% rice polish medium (pH 5.5) for 96 h at 45o C was purified to homogeneity level by sequential application of ammonium sulphate precipitation, ion exchange and gel filtratio Read More

The goal of this study was to develop a fermentation process for the production of β-glucosidase, an important enzyme in the hydrolysis of lignocellulose and has many applications in food and flavor industries, using low-cost agricultural residues as substrates. Based on statistical experimental design, high-titer production of β-glucosidase on wheat bran and glycerol by Aspergillus niger in a submerged culture was Read More