Skip to content
2000
image of Exploring the Therapeutic Potential of Bixa orellana L. Phytochemicals in Oxidative Stress and Complications Using Network Pharmacology

Abstract

Introduction

This study explores the therapeutic potential of (), focusing on its bioactive compounds and antioxidant effects. Phytochemical analysis combined with network pharmacology was employed to investigate its multi-targeted therapeutic potential against oxidative stress.

Methods

The antioxidant activity of flower extract was determined using DPPH assays. High-performance thin-layer chromatography (HPTLC) and liquid chromatography-mass spectrometry (LC-MS) were performed to profile key bioactive constituents. Network pharmacology analysis linked to these phytochemicals was performed to determine its role in oxidative stress and associated complications.

Results

The results showed a significant DPPH activity of the flower extract of . HPTLC profiling showed several major and minor metabolites at different Rf values, while LC-MS profiling revealed quercetin, kaempferol, genestin, etc, as key components of the flower extract. In network pharmacology analysis, quercetin showed a significant interaction with genes like TP53, NOS2, and CAT, while kaempferol targeted SOD2 and NFE2L2, both crucial in oxidative stress regulation. The results highlight the multi-targeted approach of flower extract compounds in modulating oxidative stress pathways.

Conclusion

This comprehensive approach revealed multiple molecular pathways influenced by compounds, providing a strong foundation for their therapeutic potential in oxidative stress-related diseases.

Loading

Article metrics loading...

/content/journals/cbc/10.2174/0115734072350859241119103503
2025-02-06
2025-04-24
Loading full text...

Full text loading...

References

  1. Gaurav I. Sharma I. Khan M.U. Zahiruddin S. Basist P. Ahmad S. Multi-mechanistic and therapeutic exploration of nephroprotective effect of traditional ayurvedic polyherbal formulation using in silico, in vitro and in vivo approaches. Biomedicines 2023 11 1 168 10.3390/biomedicines11010168 36672676
    [Google Scholar]
  2. Khan M.U. Basist P. Gaurav S. Zahiruddin S. Penumallu N.R. Ahmad S. Ameliorative effect of traditional polyherbal formulation on TNF-α, IL-1β and Caspase-3 expression in kidneys of wistar rats against sodium fluoride induced oxidative stress. J. Ethnopharmacol. 2024 318 Pt A 116900 10.1016/j.jep.2023.116900 37442489
    [Google Scholar]
  3. Haque S.E. Ekbbal R. Iqubal A. Ansari M.A. Ahmad S. Evaluation of cardioprotective potential of isolated swerchirin against the isoproterenol-induced cardiotoxicity in wistar albino rats. Pharmacogn. Mag. 2022 18 77 10 10.4103/pm.pm_500_20
    [Google Scholar]
  4. Ponnusamy S. Ravindran R. Zinjarde S. Bhargava S. Ravi Kumar A. Evaluation of traditional Indian antidiabetic medicinal plants for human pancreatic amylase inhibitory effect in vitro. Evid. Based Complement. Alternat. Med. 2011 2011 1 515647 10.1155/2011/515647 20953430
    [Google Scholar]
  5. Lans C.A. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus. J. Ethnobiol. Ethnomed. 2006 2 1 45 10.1186/1746‑4269‑2‑45 17040567
    [Google Scholar]
  6. Ekbbal R. Regulation of Interleukin 2, Proto-Oncogene c-Fos and Poly (ADP-Ribose) Glycohydrolase Genomic Expression by Rosmarinic Acid in Attenuation of Diabetes and Associated Complications. JBRHA 2023 37 6 3321 3331
    [Google Scholar]
  7. Raju S. K. Review on phytochemical composition and pharmacological activities of Bixa orellana L. J Pharm Biol Sci 2023 10 2 57 67 10.18231/j.jpbs.2022.012
    [Google Scholar]
  8. Mandal S. Das U. Studies on Diversity of Natural Dye Yielding Plants of Birbhum and Burdwan Districts of West Bengal, India. Int. J. Econ. Plants 2022 9 1 22 27 10.23910/2/2022.0433
    [Google Scholar]
  9. Ali S. Quality standards and pharmacological interventions of natural 2 oils: Current scenario and future perspectives. ACS Omega 2023 8 2 10.1021/acsomega.3c05241
    [Google Scholar]
  10. GC–MS metabolomics and network pharmacology-based investigation of molecular mechanism of identified metabolites from Tinospora cordifolia (Willd.) miers for the treatment of kidney diseases. Pharmacogn. Mag. 2022 18 79 548 558 10.4103/pm.pm_582_21
    [Google Scholar]
  11. Gaurav Khan M.U. Basist P. Zahiruddin S. Ibrahim M. Parveen R. Krishnan A. Ahmad S. Nephroprotective potential of Boerhaavia diffusa and Tinospora cordifolia herbal combination against diclofenac induced nephrotoxicity. S. Afr. J. Bot. 2022 151 238 247 10.1016/j.sajb.2022.01.038
    [Google Scholar]
  12. Gautam G. Parveen R. Ahmad S. LC-MS-based Metabolomics of Medicinal Plants. Omics Studies of Medicinal Plants 2023 157 180 10.1201/9781003179139‑9
    [Google Scholar]
  13. Salar S. Quality control and multi-targeted therapeutic approach of nyctanthes arbor-tristris for management of Hepatic disease and associated complications. Pharmacogn. Mag. 2023 2023 10.1177/09731296231189619
    [Google Scholar]
  14. Eloutify Y.T. El-Shiekh R.A. Ibrahim K.M. Elshimy R. Avula B. Katragunta K. Khan I.A. Meselhy M.R. Bioassay-Guided Isolation of Antimicrobial Components and LC/QToF Profile of Plumeria obtusa : Potential for the Treatment of Antimicrobial Resistance. ACS Omega 2023 8 7 6476 6491 10.1021/acsomega.2c06803 36844537
    [Google Scholar]
  15. Timsina B. Nadumane V.K. Purification and evaluation of bioactive fractions for anti-cancer potentials from the flowers and leaves of Nyctanthes arbor-tristis L. Warasan Khana Witthayasat Maha Witthayalai Chiang Mai 2016
    [Google Scholar]
  16. Zahiruddin S. Khan W. Nehra R. Alam M.J. Mallick M.N. Parveen R. Ahmad S. Pharmacokinetics and comparative metabolic profiling of iridoid enriched fraction of Picrorhiza kurroa – An Ayurvedic Herb. J. Ethnopharmacol. 2017 197 157 164 10.1016/j.jep.2016.07.072 27469200
    [Google Scholar]
  17. Misra M. Chauhan B. Ekbbal R. Yadav S. Gaurav N. Yasheshwar Y. Exploring antihypertensive effect of rutin through system based computational analysis. Ann. Phytomed. 2023 12 1 1 7 10.54085/ap.2023.12.1.69
    [Google Scholar]
  18. Patel M. Patel P. HPTLC Fingerprinting analysis of phytoconstituents from Bixa orellana and Beta vulgaris plant. Journal of Advanced Biotechnology and Experimental Therapeutics 2022 5 2 292 10.5455/jabet.2022.d116
    [Google Scholar]
  19. de Moura M.C.L. de Araújo V.L.L. de Sousa J.A. Pharmacokinetic, toxicological and pharmacodynamic analysis of flavonoid quercetin isolated seeds of Bixa orellana l. Research, Society and Development 2020 9 3 e170932242 10.33448/rsd‑v9i3.2242
    [Google Scholar]
  20. Cuong T.V. Chin K.B. Effects of Annatto (Bixa orellana L.) seeds powder on physicochemical properties, antioxidant and antimicrobial activities of pork patties during refrigerated storage. Han-gug Chugsan Sigpum Hag-hoeji 2016 36 4 476 486 10.5851/kosfa.2016.36.4.476
    [Google Scholar]
  21. Lima Viana J. Zagmignan A. Lima Lobato L.F. Gomes Abreu A. Jr da Silva L.C.N. Sá J.C. Monteiro C.A. Lago J.H.G. Gonçalves L.M. Carvalho R.C. Neto L.G.L. de Sousa E.M. Hydroalcoholic Extract and Ethyl Acetate Fraction of Bixa orellana Leaves Decrease the Inflammatory Response to Mycobacterium abscessus Subsp. massiliense. Evid. Based Complement. Alternat. Med. 2018 2018 1 6091934 10.1155/2018/6091934 30369954
    [Google Scholar]
/content/journals/cbc/10.2174/0115734072350859241119103503
Loading
/content/journals/cbc/10.2174/0115734072350859241119103503
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test