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image of Isolation, Characterization, and Validation of Amide Alkaloids from Piper Guineense Seeds

Abstract

Introduction

Alkaloids are naturally occurring nitrogen-containing chemicals in plants, fungi, and mammals. These chemicals protect plants from herbivores and pathogens and have a wide range of biological actions, making them useful in pharmacology and medicine. Alkaloids offer potent therapeutic effects, including antifungal, local anaesthetic, anti-inflammatory, antineoplastic, pain-killing, neuropharmacological, and antibacterial properties. Morphine is commonly used to relieve pain, quinine for malaria, and vincristine for cancer. Because of their considerable therapeutic potential, alkaloids have become a focus of study in the development of medications to treat infections, inflammation, neurological diseases, and cancer.

Objective

The primary goal of this research is to isolate, purify, and validate amide alkaloids from the seeds of PIPER guineense, a West African species while contrasting traditional alkaloid extraction methods with novel techniques such as molecular distillation, membrane separation, molecular imprinting, and high-speed counter-current chromatography. The study aims to address the challenges posed by conventional methods' high cost and low yield, by providing insights into more efficient, scalable, and advanced technologies for alkaloid extraction and purification, which are critical for the advancement of complementary and Chinese medicine.

Materials and Methods

The seeds of Piper guineense were extracted using a Soxhlet device with methanol as the solvent. The extracted extract was purified and separated into amide alkaloids using column chromatography. Thin-layer chromatography (TLC) was used to confirm the presence of alkaloids. The isolated compounds were examined and characterized using an array ofspectrum methods, including Mass spectra, FTIR, and NMR spectroscopy. Natural substances with nitrogen in the exocyclic outlook, such as mescaline, serotonin, & dopamine, were recognized as amines rather than alkaloids in the investigation.

Results

The synthesized chemicals, including Piperine, Wisanine, Piperlonguminine, Propiverine, and Piperidine, were satisfactorily characterized using FTIR, NMR, mass spectrometry, and HPLC. The FTIR spectra showed distinct peaks whichwere consistent with conventional standards, demonstrating the effective synthesis. NMR research, performed with a BRUKER AVANCE NEO-500MHz spectrometer, revealed information on the chemical surroundings of the protons, confirming the molecular structure. Mass spectroscopy was done using a MICROMASS Q-TOF micro-mass spectrometer to precisely measure molecular weights and identify fragmentation patterns, verifying the existence of important functional groups and assuring the structural integrity of the compounds.

Conclusions

This work effectively extracted, purified, and characterized numerous amide alkaloids from Piper guineense seeds using modern chromatographic and spectroscopic methods. The compounds, which included Piperine, Wisanine, Piperlonguminine, Propiverine, and Piperidine, were validated using FTIR, NMR, mass spectroscopy, and HPLC to ensure structural integrity and composition. The study reveals the efficacy of contemporary extraction and analytical procedures, offering useful insights into how to improve alkaloid purification processes. These discoveries advance our understanding of alkaloid chemistry and have the potential for future uses in alternative medicine and pharmaceutical research.

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2025-04-24

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Isolation, Characterization, and Validation of Amide Alkaloids from Piper Guineense Seeds
Bentham Science Publishers ; https://doi.org/10.2174/0115734072342018250104231522
/content/journals/cbc/10.2174/0115734072342018250104231522
/content/journals/cbc/10.2174/0115734072342018250104231522
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  • Article Type:     Research Article
Keywords: purification ; Extraction ; characterization ; alkaloids ; separation ; phytoconstituents

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