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image of Trafficking of Muscarinic 1 Acetylcholine Receptor Regulated by VPS35 in Alzheimer's Disease

Abstract

Introduction

Muscarinic 1 acetylcholine receptor (M1AChR) is a member of the G-protein-coupled receptor superfamily, with the dysfunction being linked to the onset of Alzheimer's Disease (AD).

Aims

Retromer complex with Vacuolar Protein Sorting-35 (VPS35) as the core plays an important role in the transport of biological proteins and has been confirmed to be closely related to the pathogenesis of AD. This study was designed to determine whether VPS35 could affect the trafficking mechanism of M1AChRs.

Method

The interaction between VPS35 and M1AChR was studied by co-immunoprecipitation method, and the recycling of M1AChR influence by VPS35 was analyzed using biotinylation technology.

Results

It was found that VPS35 affected the localization of M1AChR on the cell membrane by regulating intracellular M1AChR transport, thus controlling the M1AChR-mediated cholinergic signaling pathway.

Conclusion

The findings presented here provide a potential pathogenesis and pathway for the treatment of AD.

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2025-01-28
2025-03-27

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/content/journals/car/10.2174/0115672050356990250111200217
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Trafficking of Muscarinic 1 Acetylcholine Receptor Regulated by VPS35 in Alzheimer's Disease
Bentham Science Publishers ; https://doi.org/10.2174/0115672050356990250111200217
/content/journals/car/10.2174/0115672050356990250111200217
/content/journals/car/10.2174/0115672050356990250111200217
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  • Article Type:     Research Article
Keywords: retromer complex ; trafficking ; muscarinic 1 acetylcholine receptors ; Alzheimer's disease ; vacuolar protein sorting-35

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