Current Analytical Chemistry - Volume 6, Issue 3, 2010

Several fluorescence reagents (i.e., Fmoc-Cl, DMEQ-COCl, DBD-F, PSC and DNS-Cl), which are reactive with an amino functional group, were evaluated for the labeling of the β-glycosylamine moiety in a glycoprotein. Because the β-glycosylamine is released from a glycoprotein containing asparaginyl-oligosaccharides (N-linked oligosaccharide) with glycoamidase F (PNGase F), the enzyme amount and the reaction time whic Read More

Biocatalysis in nonaqueous media currently constitutes a promising research area which has already demonstrated its potential in numerous and varied applications. Several possibilities exist for the analysis of a wide range of poorly water-soluble analytes present in samples of food, pharmaceuticals, petrochemicals and cosmetic products, among others. The idea of assembling this kind of biocatalysis with flow mana Read More

Indole-3-carboxaldehyde (In3C) molecules were deposited on the glassy carbon (GC) electrode by electrochemical methods in acetonitrile (containing 0.05 M TBATFB). Deposition of In3C was performed by cyclic voltammetry scanning from 0.0 V to 2.0 V at a scan rate of 50 mV s-1 for 10 cycles in 1 mM In3C solution. The modified surface (In3C-GC) was characterized by cyclic voltammetric response of ferrocene as a re Read More

The S-(-)-enantiomer of ofloxacin, a chiral fluoroquinolone antibiotic, exhibits an antibacterial activity 8-128 times higher than that of the R-(+)-isomer. A new stereospecific nano-LC method for the enantioseparation of the ofloxacin racemic mixture was developed using an achiral reversed phase column (Pinnacle II Phenyl 3 μm, 15 cm x 100 μm ID) and heptakis-(2,3-diacetyl-6-sulfo)-β-cyclodextrin (HDAS-β-CD) as a mobile p Read More

In this work results are reported on the determination of some elements, Ni, Sn, Te and Zn, present at ultratrace levels (ng g-1) in three different samples of metallurgical lead, i.e. electrolytically refined lead, thermally refined lead and lead with added impurities, assigned to be employed as standard reference materials by BCR. The Instrumental Neutron Activation Analysis was used for its characteristics to be a primary anal Read More

The quality assessment of water in term of organometallic compounds is one of the key tasks of modern environmental analytical chemistry. For this purpose a rapid and very sensitive method based on solid-phase microextraction (SPME) followed by gas chromatography-inductively coupled plasma-mass spectrometry (GC-ICP-MS) was developed and validated aimed at the determination of dimethyl-, dibutyl- and tributyltin in Read More

A simple, accurate and sensitive kinetic method has been developed for the determination of three cardiovascular drugs: Betaxolol, Clopidogrel, and Imidapril in pharmaceutical formulations. The method is based on the alkaline oxidation of the selected drugs with potassium manganate(VII) at room temperature (25°C) to produce a bluish-green colored product. The reaction is followed spectrophotometrically by measuring t Read More

This article presents an overview of the different analytical methods available for performing pesticide degradation studies in water and environmental samples by different processes. How long the pesticide remains in the environment depends on how strongly it is bounded by other components and how readily it is degraded. It is also depends on the environmental conditions at the time of application, pH, temperatu Read More

Ionic liquid has been rapidly developed as an environment-friendly acceptor phase for microextraction and sample preparation in analytical chemistry in recent years. Present microextraction with ionic liquid mainly includes liquid phase microextraction, single drop liquid phase microextraction, solid phase microextraction, dispersive liquid phase microextraction and cold induced aggregation microextraction. In Read More