Current Analytical Chemistry - Volume 12, Issue 1, 2016

This review introduces the concept of enzyme biosensors. As the major feature of an enzyme is its selectivity and specificity, we briefly highlighted the principles of enzyme production and enzyme classification. Moreover, enzyme immobilization methods, including physical adsorption and entrapment techniques with special attention on cross-linking and covalent binding are discussed followed by different electrochemical Read More

Mercury is one of the most toxic elements that may be found in natural water bodies and can negatively affect living organisms, human health as well as drinking water quality. In addition, water is the main transportation and transformation pathway of mercury into the environment and living organisms. Therefore, specific strategies have been developed to reduce mercury releases and to remediate mercury contaminated ar Read More

An overview on the progress in the organophosphorus hydrolase based electrochemical sensors design and application for direct organophosphorus pesticides quantification is presented in this work. The key analytical performances of the enzyme and of the bacterial potentiometric and amperometric organophosphorus hydrolase based sensors, as well as their advantages and deficiencies are comme Read More

This paper presents a new disposable electrochemical biosensor for the analysis of Haptoglobin (Hp) which shows many important functionalities such as antioxidant capabilities, antiinflammatory activities and regulation of the immune system. Aminopropyltriethoxysilane (APTES) was self-assembled onto an indium tin oxide (ITO) substrate to fabricate a disposable biosensor. Anti- Hp antibodies were immobilized onto the m Read More

In this work, an enzymatic biofuel cell (BFC) was developed by combining nanomaterial modified and plain glassy carbon paste electrodes (GCPE). Because of the composite nature of GCPE, very practical and economic bio-anode and bio-cathode electrodes were formed. Bio-anode electrode was obtained by modification of composite GCPE with glucose oxidase (GOx) and gold nanoparticle (Au np). The effects of various nano Read More

Hydrophilic interaction liquid chromatography (HILIC) has been used to investigate the retention characteristics of quercetin and its glycosides typically present in plant samples. Two stationary phases – bare silica and with zwitterionic sulfoalkylbetaine groups on silica surface (ZIC) were used. The effect of acetonitrile content and the presence of salt in the mobile phase were studied. The analyte retention factors were ob Read More

The use of immobilized enzymes in proteomics studies has increased over the last decade not only due to their ease of use in fluidic systems but also because autoproteolysis is suppressed allowing use of high ratios of enzyme-to-substrate for rapid digestion. Chymotrypsin immobilization by crosslinking with excess aqueous glutaraldehyde (GA) has been used to produce a gel-like agglomerated pellet of high enzyme loading c Read More

Melamine was quantified in liquid milk without performing the usual liquid-liquid or solidphase extraction steps. Following a simple clean up procedure (extraction by acetonitrile/water mixture, centrifugation and filtration by 0.45 μm membrane), melamine was accurately quantified in fullfat liquid milk down to 0.057 mg kg-1 using second order multivariate calibration. Second order data was created from absorbance-p Read More