Current Analytical Chemistry - Volume 10, Issue 2, 2014

Capillary Electrophoresis (CE) has demonstrated its wide potential to solve many analytical problems. Recent developments in CE with contributions for sample preparation, improvements in detection and aspects associated with application and productivity requirements in routine laboratories are reviewed and discussed. Although these capabilities of CE, the transference of CE methods to routine analytical work is very limited. Read More

An on-line preconcentration capillary electrophoresis (CE) methodology, which combines a normal stacking mode (NSM) with a dynamic pH junction technique, is introduced in this paper. A systematic study of the parameters (concentration and pH value of background electrolyte, composition of sample matrix, injection pressure and time, separation voltage and temperature) affecting on-line concentration of seven related pheno Read More

A rapid and simple analytical capillary zone electrophoresis (CZE) method has been developed and validated for quantitative determination of atypical antipsychotic drug risperidone in commercially available film-coated tablets. CZE was performed in an uncoated fused-silica capillary 40.2 cm total length x 75 μm i.d. (30 cm effective length) using a 20 mmol mL-1 sodium phosphate solution containing 0.1% triethylamine (v/ Read More

A rapid and simple microemulsion electrokinetic chromatography (MEEKC) method has been developed and validated for the determination of ethinylestradiol and levo-norgestrel in a commercial tablet formulation. The analysis was performed with an electrolyte composed of 0.5% (w/w) ethyl acetate, 1.2% (w/w) butan-1-ol, 0.6% (w/w) sodium dodecyl sulfate, 15% (v/v) ethanol and 82.7% (w/w) 12 mmol L-1 sodium tetrab Read More

This article focuses on the main developments in element speciation analysis by capillary electrophoresis (CE) since the 1990s. A brief description of the main techniques employed in CE analysis has been reviewed with emphasis in the chemical species derived from inorganic forms and organic compounds bound to metal and non-metal elements. Analysis of the most important elemental species existing in the environm Read More

A capillary zone electrophoretic (CZE) method has been developed for the determination of zoledronate and its related impurities (phosphyte and phosphate). Successful separation of the drug from the impurities was achieved using 7.5 mM of phthalic acid adjusted to pH 3.50 with TRIS, as background electrolyte with an indirect detection at 205 nm. The optimized method was validated for specificity, precision, linearity and Read More

An estrogen selected molecular imprinted polymer (MIP) has been developed to be used as an alternative sorbent in solid phase extraction of serum samples before capillary electrophoresis analysis. Following a heat polymerization, MIP has been synthesized using methacrylic acid (MAA) as functional monomer, ethyleneglycol dimethacrylate (EGDMA) as crosslinker, benzoyl peroxide as radical initiator and 17β- estradiol as Read More

Optimization and validation of a capillary zone electrophoresis (CZE) method for the determination of inner and external inorganic sulfate from doxorubicin-loaded liposomes as a measurement of encapsulation efficiency are presented. Sulfate was determined using a fused silica capillary (50 cm x 75 μm ID) and background electrolyte (BGE) composed of 5 mM chromate with 1 mM of TTAB at pH 10.5 with indirect UV dete Read More

Capillary electrophoresis (CE) with organic solvents has gained special interest in pharmaceutical analysis as it offers an alternative to overcome problems with the solubility especially of lipophilic analytes in aqueous solutions. However, beside the solubility of the compounds (typical pharmaceuticals are weak acids and bases, that can be present in ionic and molecular form as well) other properties and variables relev Read More

Extremely good enantioseparation of a kind of commonly used β-blockers, (±)-propranolol, has been achieved on silica gel layers which were impregnated with β-cyclodextrin and oxidized multi-walled carbon nanotubes. Solvent system tert-butenol-acetonitrile (1:1, v/v) successfully resolved the enantiomers of (±)-propranolol. The spots were detected at 254 nm with a UV lamp. The effect of concentration of the mo Read More

Salicylic acid (SA) plays a key role against pathogens of plants and also induces resistance, hence it is an important signaling compound in plants. SA derivative, such as acetylsalicylic acid (Aspirin®), is widely used and marketed today as a non steroidal anti-inflammatory drug (NSAIDs) which is one of the first “wonder drugs” of the 20th century. Here we report a method for quantification of SA and its derivatives i.e. 3,4-dihyroxy Read More

Dispersive liquid–liquid microextraction (DLLME) coupled with high-performance liquid chromatography (HPLC) equipped with UV detector was used for the extraction and determination of efavirenz in plasma sample. Acetonitrile and chloroform were used as disperser and extraction solvents, respectively. Several parameters, including extraction solvent, the type of dispersion solvent, volume of extraction and dispersion solven Read More

Silica gel was modified via silanization with 3-chloropropyltrimethoxysilane and subsequently Cu(II)-5- dodecylsalicylaldoxime was grafted. Obtained sorbent was applied to on-line copper(II) preconcentration and its FAAS determination. Effective copper(II) elution was observed for 2% v/v nitric acid. The new sorbent exhibits optimal sorption efficiency within pH 5.6 and 7.5. A portion of the sorbent may be used repeatedly for not Read More