Immobilised Metal Affinity Chromatography for the Analysis of Proteins and Peptides

Gushinder Kaur-Atwal; Daniel J. Weston; Philip L.R. Bonner; Susan Crosland; Philip S. Green; Colin S. Creaser

doi:10.2174/157341108784587777

ISSN: 1573-4110
E-ISSN: 1875-6727

Immobilised Metal Affinity Chromatography for the Analysis of Proteins and Peptides
By Gushinder Kaur-Atwal, Daniel J. Weston, Philip L.R. Bonner, Susan Crosland, Philip S. Green and Colin S. Creaser
Source: Current Analytical Chemistry, Volume 4, Issue 2, Apr 2008, p. 127 - 135
DOI: https://doi.org/10.2174/157341108784587777
- Available online: 01 Apr 2008

Abstract

Immobilised metal affinity chromatography (IMAC) has become an established purification procedure for the selective isolation of proteins, peptides and post-translationally modified peptides, using a wide range of metal ions, including Cu(II), Fe(III) and Ga(III). Recently, the potential of interfacing capillary IMAC with mass spectrometry has been explored. This article reviews the development and use of IMAC in protein/peptide analysis, highlighting the benefits of capillary columns and their application to IMAC based enrichment.

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2008-04-01

2025-04-18

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Article Type: Research Article

Keyword(s): Capillary column; Immobilised metal affinity chromatography; Liquid chromatography-mass spectrometry; Protein/peptide analysis; Selective isolation

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Immobilised Metal Affinity Chromatography for the Analysis of Proteins and Peptides

Abstract

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