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Background: In this study, trace copper plays an important role in plant growth and is a biological component of various enzymes involved in both photosynthesis and plant respiration. Objective: Electrochemical voltammetric bioassay of trace copper ion was detected using photo-sensing diode circuits (PSD) with working sensors for ppb levels trace detection in agricultural water by stripping and cyclic voltammetry. Results: Optimum voltammetric conditions were examined. The system used was simple and utilized inexpensive circuits of our fabricated systems; however, a low detection limit was attained at 40.0 ugL-¹. Conclusion: The developed technique can be used in determining trace amounts of copper, especially in agricultural water resources.