Current Analytical Chemistry - Volume 1, Issue 2, 2005

During long-term treatment with STI-571 (Gleevec®, Glivec®, Imatinib), it is possible that tumor cells change their expression of proteins in order i.a. to reduce their exposure to STI-571. This would eventually result in reduced activity. In order to evaluate this possibility, we have developed an analytical technique to determine intrinsic cellular concentrations of STI-571 in tumor cells which have been manipulated to generate lin Read More

An efficient method for the determination of formaldehyde in frozen fish by SPME-gas chromatography-ion trap tandem mass spectrometry was developed and validated. Experimental design was used to investigate the effects of temperature of extraction, time of extraction and matrix stirring. The calculated regression model was used to find the experimental conditions providing the optimal SPME extraction yield. Valid Read More

Since its introduction over 60 years ago, gas chromatography (GC) has become a cornerstone technique of analytical chemistry. While the basic components of a GC instrument have not changed since its introduction by Martin and James in 1952, instrumentation refinement has answered demands for faster, more sensitive and selective separations of complex samples. This review will discuss the advances made in GC inst Read More

The basic principles, instrumental developments and applications of dual-opposite injection capillary electrophoresis (DOI-CE) technique are reviewed. Two different dual-opposite injection modes are described: electrokinetic injection and sequential hydrodynamic injection. The features of both injection principles are compared, followed by a detailed discussion on the biases of electrokinetic injection and how to reduce the Read More

In this paper we review a series of water-soluble quinolinium boronic acid fluorescent probes, sensitive to halide and cyanide anions. We have intelligently combined the well-known halide, in particular chloride, bromide and iodide, sensing capability of the quinolinum nucleus with the fluoride or cyanide ion binding boronic acid moiety, to afford a range of water-soluble probes sensitive to fluoride, chloride, bromide, iod Read More

A new methodology is proposed for arsenic, phosphorus and titanium determination in crystalline lamellar phenylarsonate and phenylphosphonate samples by an ICP OES technique. Nitric acid, hydrofluoric acid and hydrogen peroxide were used to decompose mass aliquots of about 50 mg of titanium phenylphosphonate and phenylarsonate in a closed microwave oven. This method was shown to be suitable, reproducible and s Read More

A new droplet method has been developed for the determination of nitrogen dioxide in air. The method based on the reaction of nitrite with sulfathiazole in acidic medium and then coupled with NEDA (N naphthylethylenediamine dihydrochloride). The reaction product (azo dye) has an absorption maximum at 546 nm . The developed system describe the coupling of the sampling device behavior of the liquid droplet usin Read More

The detection of losses from nuclear material inventories whether by theft, leakage or other means is a problem of considerable importance. The earliest approaches to this problem were based on control chart like methods that made the assumption that the time series of material balance measurements (material balances are computed directly from analytical measurements of the amount of material present in such a ma Read More

Over the past 30 years, tremendous efforts have been made for the development of a safe, costeffective and efficient device for nucleic acid delivery to mammalian cells. The approach has great contribution to analysis of gene expression, function and regulation and production of numerous recombinant therapeutic proteins, as well as bright prospects for human gene therapy. Of the existing techniques either base Read More

The purpose of this work is to present a peer revision to the last trends dealing with ultrasonication for sample treatment in Analytical Chemistry. The selective oxidation properties of sonication makes this tool a powerful help to distinguish, when present in solution, some metal organic species from the inorganic ones (organic mercury and inorganic mercury) or to differentiate in biological matrices non-toxic from toxic metal or Read More

During recent years, arable land used for transgenic crops has undergone an exponential increase globally, and the tendency to augment not only the cultivated area but also the number of modified genetic traits is rather evident. However, public perception and confidence regarding genetically modified organisms (GMOs) have not paralleled this increase. Moreover, a general antipathy towards GMOs is nowadays det Read More