Recent Patents on Biotechnology - Online First

Harmful microorganisms like pathogens significantly impact human health. Meanwhile, industrial growth causes pollution and water contamination by releasing untreated hazardous waste. Effective treatment of these microorganisms and contaminants is essential, and nanocomposites may be a promising solution. The present attempt demonstrates the green synthesis of α-Fe2O3@TiO2 nanocomposites (FTNCs) for the effective treatment of pathogens and organic contaminants.

The α-Fe 2O3@TiO2nanocomposites (FTNCs) has been synthesized through a green approach utilizing curcumin extract. Curcumin (Turmeric) extract (TEx) was prepared by washing, drying, and crushing 5 g of turmeric, then boiling it in 100 mL distilled water at 70°C for 1 hour. Metal salts (Fe³⁺/Ti⁴⁺, 2:1) were added to 100 mL of TEx under continuous stirring at 70°C for 24 h. The solution was rinsed and dried at 80°C overnight and heated at 300°C for 3 h to remove impurities.

Synthesized FTNCs have been tested for the potent antibacterial activity against both Gram-positive (Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis) and Gram-negative bacteria (Escherichia coli, Salmonella Abony, Pseudomonas sp.). Observations discovered noteworthy inhibition of both Gram-positive and Gram-negative bacteria by FTNCs. Furthermore, the FTNCs system shows the energy band gap of ~2.6 eV which may suppress electron recombination, thereby enhancing photocatalysis and examined against Evans blue (EB) and Congo red (CR) dyes under UV and visible light (125 W) irradiation. The remarkable photocatalytic degradation efficiency (DE) for CR reached ~67.4% in 60 min.

A simple green approach has been demonstrated for the synthesis of the FTNCs using curcumin-mediated reduction. As prepared FTNCs have been evaluated for potent antibacterial activity against both Gram-positive (Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis) and Gram-negative bacteria (Escherichia coli, Salmonella Abony, Pseudomonas sp.). Observations. The results show that the highest ZID values have been obtained for 5 mg/mL concertation of FTNCs of ~14, 22,18, 21, and 20 and 29 mm for E. coli, S. abony, S. aureus, B. subtilis, E. faecalis, and Pseudomonas sp., respectively. Additionally, FTNCs demonstrate remarkable photocatalytic degradation efficiency against EB and CR dyes under UV (125 W) irradiation, achieving 56, 67% degradation within 60 minutes for EB and CR. The findings suggest that the FTNCs hold promise for long-term antimicrobial efficacy against various bacteria and offer the potential for addressing water and wastewater contaminants through photocatalysis.