Skip to content
2000
  1. Home
  2. A-Z Publications
  3. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry - Anti-Cancer Agents)
  4. Volume 24, Issue 17
  5. Article
Volume 24, Issue 17
  • ISSN: 1871-5206
  • E-ISSN: 1875-5992

Abstract

Introduction

Globally, about 8.2 million cancer-related deaths are recorded annually. Sadly, most of the deaths result from the toxicity of most chemotherapeutic agents. Hence, there are growing demands for chemotherapeutic agents with high specificity and selectivity. This study was designed to assess the cytotoxic potential of and isolate cytotoxic compounds with better selectivity profiles.

Methods

Stem bark (DMS) was collected and authenticated at the Forest Herbarium Ibadan (FHI), and a voucher (FHI-111954) was issued. Dried DMS was pulverized and extracted into 70% methanol. The extract was partitioned into hexane, dichloromethane, and ethyl acetate fractions. The cytotoxicities of the extract, fractions, and isolated compounds were determined. The cytotoxicity of the isolated compounds was tested against different cell lines, including human breast (AU565 and MDA MB231), oral adenosquamous (CAL27), and cervical (HeLa) cancer cells, as well as healthy (3T3) non-cancer cells.

Results

Methyl gallate, eriodictyol, quercetin, quebrachitol, catechin, catechin gallate, and gallic acid, isolated from dichloromethane and ethyl acetate fractions, displayed weak cytotoxicity against breast (AU565 and MDA-MD-231) and cervical (HeLa) cancer cell lines. Interestingly, all the compounds, except gallic acid (48.91±4.51% inhibition), displayed potent cytotoxicity on oral cancer cells. Methyl gallate and quercetin displayed the highest activity, with IC values of 89.57±1.98µM and 78.19±1.49µM, respectively. Interestingly, all the compounds were not toxic to healthy non-cancer (3T3) cells.

Conclusion

The compounds displayed anticancer activity specific to oral cancer cells and were highly selective for cancer cells without causing significant toxicity to healthy non-cancer cells.

© 2024 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/acamc/10.2174/0118715206317259240722113046
2024-10-01
2025-04-19

  • From This Site

    /content/journals/acamc/10.2174/0118715206317259240722113046
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. ChhikaraB.S. ParangK. Global Cancer Statistics 2022: the trends projection analysis.Chem. Biol. Let.2023101451
     [Google Scholar]
  2. SungH. FerlayJ. SiegelR.L. LaversanneM. SoerjomataramI. JemalA. BrayF. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries.CA Cancer J. Clin.202171320924910.3322/caac.21660 33538338
     [Google Scholar]
  3. ObafemiF.A. Umahi-OttahG. A review of global Cancer prevalence and therapy.J. Canc. Res. Treat. Prevent.20231312814710.37191/Mapsci‑JCRTP‑1(3)‑011
     [Google Scholar]
  4. WuJ. LiY. HeQ. YangX. Exploration of the use of natural compounds in combination with chemotherapy drugs for tumor treatment.Molecules2023283102210.3390/molecules28031022 36770689
     [Google Scholar]
  5. LustbergM.B. KudererN.M. DesaiA. BergerotC. LymanG.H. Mitigating long-term and delayed adverse events associated with cancer treatment: implications for survivorship.Nat. Rev. Clin. Oncol.202320852754210.1038/s41571‑023‑00776‑9 37231127
     [Google Scholar]
  6. MaS.C. ZhangJ.Q. YanT.H. MiaoM.X. CaoY.M. CaoY.B. ZhangL.C. LiL. Novel strategies to reverse chemoresistance in colorectal cancer.Cancer Med.20231210110731109610.1002/cam4.5594 36645225
     [Google Scholar]
  7. MalabadiR.B. SadiyaM. KolkarK.P. MammadovaS.S. ChalannavarR.K. BaijnathH. Role of Plant derived-medicine for controlling cancer.Int. J. Sci. Res. Arch.202411125022539
     [Google Scholar]
  8. SalawuKM. WangY. MaharjanR. AjaiyeobaEO. Antibacterial screening and isolation of compounds from Detarium microcarpum stem bark against methicillin resistant Staphylococcus aureus.J. Sci. Pract. Pharm.20207140040510.47227/jsppharm.v7i1.7
     [Google Scholar]
  9. AhmedM.E. AbdelgadirA.A. AhmedE.M. Medicinal and aromatic plants from sudan: Traditional uses, pharmacology, and phytoconstituents. In: Plants as Medicine and Aromatics.CRC Press2023457410.1201/9781003226925‑5
     [Google Scholar]
  10. SaiduI.N. UmarK.S. IsaM.H. Ethnobotanical survey of anticancer plants in Askira/Uba local government area of Borno State, Nigeria.Afr. J. Pharm. Pharmacol.20159512313010.5897/AJPP2014.4083
     [Google Scholar]
  11. SalawuK. OgboleO. AbiodunO. AjaiyeobaE. Ethnobotanical survey, phytochemical screening, growth inhibitory effects and cytotoxicity evaluation of medicinal plants used for cancer management in ilorin metropolis, Nigeria.Arch. Basic Appl. Med.202192168175
     [Google Scholar]
  12. KritsanawongS. InnajakS. ImotoM. WatanapokasinR. Antiproliferative and apoptosis induction of α-mangostin in T47D breast cancer cells.Int. J. Oncol.20164852155216510.3892/ijo.2016.3399 26892433
     [Google Scholar]
  13. EkaprasadaM.T. NurdinH. IbrahimS. DachriyanusD. Antioxidant activity of methyl gallate isolated from the leaves of Toona sureni.Indon. J. Chem.20109345746010.22146/ijc.21515
     [Google Scholar]
  14. MandalN. ChaudhuriD. GhateN.B. SinghS.S. Methyl gallate isolated from Spondias pinnata exhibits anticancer activity against human glioblastoma by induction of apoptosis and sustained extracellular signal-regulated kinase 1/2 activation.Pharmacogn. Mag.2015114226927610.4103/0973‑1296.153078 25829764
     [Google Scholar]
  15. Atta-ur-Rahman MICaA-t-W. Solving Problems with NMR Spectroscopy.2nd edAcademic Press2016
     [Google Scholar]
  16. Yu-xianLi. YuanKe. Isolation and structural elucidation of chemical constituents of Mussaenda hainanensis Merr.J. Med. Plants Res.20115814591465
     [Google Scholar]
  17. MoriK. Pheromone synthesis. Part 253: Synthesis of the racemates and enantiomers of triglycerides of male Drosophila fruit flies with special emphasis on the preparation of enantiomerically pure 1-monoglycerides.Tetrahedron201268408441844910.1016/j.tet.2012.07.086
     [Google Scholar]
  18. DíazM. GonzálezA. Castro-GamboaI. GonzalezD. RossiniC. First record of l-quebrachitol in Allophylus edulis (Sapindaceae).Carbohydr. Res.2008343152699270010.1016/j.carres.2008.07.014 18715552
     [Google Scholar]
  19. DandareA. ZarewaS. AbdullahiK. RabiuS. OnwugharaC. Amilioretive effect of aqueous leaves extract of Ziziphus mucronata in ethanol induced gastric ulcer model rats. J. Appl.Life Sci. Int.20171521810.9734/JALSI/2017/37862
     [Google Scholar]
  20. DavisA.L. CaiY. DaviesA.P. LewisJ.R. 1H and 13C NMR assignments of some green tea polyphenols.Magn. Reson. Chem.1996341188789010.1002/(SICI)1097‑458X(199611)34:11<887:AID‑OMR995>3.0.CO;2‑U
     [Google Scholar]
  21. SarriaV.R.A. CorredorJ.A.G. IsabelP.M. Isolation of catechin and gallic acid from Colombian bark of Pinus patula.Chem. Sci. J.20178417410.4172/2150‑3494.1000174
     [Google Scholar]
  22. OgboleO.O. SegunP.A. AdenijiA.J. In vitro cytotoxic activity of medicinal plants from Nigeria ethnomedicine on Rhabdomyosarcoma cancer cell line and HPLC analysis of active extracts.BMC Complement. Altern. Med.201717149410.1186/s12906‑017‑2005‑8 29166892
     [Google Scholar]
  23. KamathamS. KumarN. GudipalliP. Isolation and characterization of gallic acid and methyl gallate from the seed coats of Givotia rottleriformis Griff. and their anti-proliferative effect on human epidermoid carcinoma A431 cells.Toxicol. Rep.2015252052910.1016/j.toxrep.2015.03.001 28962387
     [Google Scholar]
  24. AnjumS. GaniA. AhmadM. ShahA. MasoodiF.A. ShahY. GaniA. Antioxidant and antiproliferative activity of walnut extract (Juglans regia L.) processed by different methods and identification of compounds using GC/MS and LC/MS technique.J. Food Process. Preserv.2017411e1275610.1111/jfpp.12756
     [Google Scholar]
  25. LiM. YangJ. ZhangL. TuS. ZhouX. TanZ. ZhouW. HeY. LiY. A low-molecular-weight compound exerts anticancer activity against breast and lung cancers by disrupting EGFR/Eps8 complex formation.J. Exp. Clin. Cancer Res.201938121110.1186/s13046‑019‑1207‑y 31118055
     [Google Scholar]
  26. KimB.W. LeeE.R. MinH.M. JeongH.S. AhnJ.Y. KimJ.H. ChoiH.Y. ChoiH. KimE.Y. ParkS.P. ChoS.G. Sustained ERK activation is involved in the kaempferol-induced apoptosis of breast cancer cells and is more evident under 3-D culture condition.Cancer Biol. Ther.2008771080108910.4161/cbt.7.7.6164 18443432
     [Google Scholar]
  27. LinJ.N. LinH.Y. YangN.S. LiY.H. LeeM.R. ChuangC.H. HoC.T. KuoS.C. WayT.D. Chemical constituents and anticancer activity of yellow camellias against MDA-MB-231 human breast cancer cells.J. Agric. Food Chem.2013614010.1021/jf4029877 24001127
     [Google Scholar]
  28. GovindanS.V. KulsumS. PandianR.S. DasD. SeshadriM. HicksW.Jr KuriakoseM.A. SureshA. Establishment and characterization of triple drug resistant head and neck squamous cell carcinoma cell lines.Mol. Med. Rep.20151223025303210.3892/mmr.2015.3768 25962396
     [Google Scholar]
  29. TakaraK. HoribeS. ObataY. YoshikawaE. OhnishiN. YokoyamaT. Effects of 19 herbal extracts on the sensitivity to paclitaxel or 5-fluorouracil in HeLa cells.Biol. Pharm. Bull.200528113814210.1248/bpb.28.138 15635178
     [Google Scholar]
  30. HuangC.Y. JuD.T. ChangC.F. Muralidhar ReddyP. VelmuruganB.K. A review on the effects of current chemotherapy drugs and natural agents in treating non–small cell lung cancer.Biomedicine 2017742310.1051/bmdcn/2017070423 29130448
     [Google Scholar]
  31. TuncayS. SenolH. GulerE.M. OcalN. SecenH. KocyigitA. TopcuG. Synthesis of oleanolic acid analogues and their cytotoxic effects on 3T3 cell line.Med. Chem.201814661762510.2174/1573406414666180222094544 29473521
     [Google Scholar]
  32. ChanC.Y. WeiL. Castro-MuñozledoF. KooW.L. (−)-Epigallocatechin-3-gallate blocks 3T3-L1 adipose conversion by inhibition of cell proliferation and suppression of adipose phenotype expression.Life Sci.20118921-2277978510.1016/j.lfs.2011.09.006 21978785
     [Google Scholar]
  33. YahagiT. YakuraN. MatsuzakiK. KitanakaS. Inhibitory effect of chemical constituents from Artemisia scoparia Waldst. et Kit. on triglyceride accumulation in 3T3-L1 cells and nitric oxide production in RAW 264.7 cells.J. Nat. Med.201468241442010.1007/s11418‑013‑0799‑3 24142543
     [Google Scholar]
/content/journals/acamc/10.2174/0118715206317259240722113046
Loading
Isolation and Characterization of Cytotoxic Compounds from Detarium microcarpum Guill. and Perr. Stem Bark
Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry - Anti-Cancer Agents) 24, 1295 (2024); https://doi.org/10.2174/0118715206317259240722113046
/content/journals/acamc/10.2174/0118715206317259240722113046
/content/journals/acamc/10.2174/0118715206317259240722113046
Loading

Data & Media loading...

Supplements

file format download Download

  • Article Type:     Research Article
Keyword(s): cytotoxicity; Detarium microcarpum; healthy (3T3) non-cancer cells; methyl gallate; oral cancer; quercetin

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test