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Volume 4, Issue 1
  • ISSN: 2950-5704
  • E-ISSN: 2950-5712

Abstract

Background

is the most widely distributed snake in Venezuela, causing the majority of snakebite envenoming.

Objective

The purpose of this study was to produce IgY antibodies against a venom pool from different Venezuelan regions and evaluate their neutralization capacity on various venom toxic activities.

Methods

Anti- venom antibodies are purified from chicken egg yolks by precipitation with polyethylene glycol and further analyzed by Multiple Antigen Blot Assay, indirect ELISA, Western blot, and Inhibition assays. In addition, we evaluate the phospholipase, edematogenic, and hemorrhagic activities. In addition, a new envenoming simulation study using anti- venom IgY in mice is presented.

Results

In this study, we show that anti- venom IgY is capable of neutralizing 4 LD doses of the venom (., 1.76 mg of IgY neutralized 14 µg of venom) and effectively neutralizing the phospholipase, edematogenic and hemorrhagic activities. Additionally, the anti venom IgY specifically recognizes polypeptide bands with apparent molecular masses of ~ 54.55, 30.39, 24.1, 14.02, and 9.44 kDa by western blot. The IgY specificity is demonstrated by a dose-dependent inhibition, in which antibodies pre-adsorbed with the venom does not recognize the proteins contained in the venom. Furthermore, in the simulation study of envenoming, the mice inoculated with IgY showed no response.

Conclusion

Our results support the use of anti-venom IgY as an alternative to traditional equine therapy in animals and, eventually, in human patients bitten by snakes.

© 2024 Bentham Science Publishers
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2024-01-01
2024-11-26

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Pre-clinical Efficacy and Immunogenicity of IgY Antibodies Directed against Crotalus durissus cumanensis Venom
J. Curr. Toxicol. Venomics 4, E280524230401 (2024); https://doi.org/10.2174/0126661217296708240506074324
/content/journals/jctv/10.2174/0126661217296708240506074324
/content/journals/jctv/10.2174/0126661217296708240506074324
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  • Article Type:     Research Article
Keyword(s): Crotalus durissus cumanensis; hemorrhagic activities; IgY antivenom; Rattlesnakes; Venezuela; venom activities; venom pool
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