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image of Influence of Multidrug Resistance 1 Gene Variants on Response to Intravenous Methylprednisolone Pulse in Systemic Lupus Erythematosus Patients: Preliminary Results

Abstract

Introduction/Objectives

Genetic variations could explain individual responses to drugs. This case-control study aimed to investigate the association between the multidrug resistance 1 ( gene exonic single nucleotide variants (SNVs), and , and the response to intravenous methylprednisolone in Egyptian patients with active systemic lupus erythematosus (SLE).

Method

Real-time polymerase chain reaction was used. Patients were divided into responders and resistant based on the SLE Disease Activity Index (SLEDAI). The degree of improvement was determined according to a 7-point Likert scale.

Results

The study included 80 patients: 40 patients with renal flares and 40 patients with extrarenal flares. In patients with extrarenal flares, 71.4% of responders had the CT+TT model of the variant 36.8% of resistant patients ( = 0.028); the T allele was detected in 47.6% of responders 23.7% of resistant patients ( = 0.026). Patients with the TT and CT genotypes, TT+CT model, and T allele of the variant had significant improvement based on the Likert scale compared with the CC genotype, CC model and C allele ( = 0.049, 0.038, 0.010 and <0.001, respectively). In the renal subgroup, patients with the CC genotype and C allele of the variant had significant improvement based on the Likert scale compared with the CT genotype and T allele ( = 0.028 and 0.046, respectively). Patients with the CC model had significantly lower post-treatment proteinuria compared with the TT+CT model ( = 0.024).

Conclusion

MDR1 C1236T variant allele and C3435T wild allele seem to enhance the response to glucocorticoids in Egyptian patients with active SLE.

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2025-01-08
2025-05-07

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Influence of Multidrug Resistance 1 Gene Variants on Response to Intravenous Methylprednisolone Pulse in Systemic Lupus Erythematosus Patients: Preliminary Results
Bentham Science Publishers ; https://doi.org/10.2174/0115733971345970241107114732
/content/journals/crr/10.2174/0115733971345970241107114732
/content/journals/crr/10.2174/0115733971345970241107114732
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Supplements

Supplementary material is available on the publisher's website along with the published article. Supplementary Material 1: A flow chart for the selection and enrollment of patients Supplementary Material 2: Detailed genotyping and Real Time PCR techniques. Table Demographics, comorbidities, disease characteristics and system-based disease flares of the study cohort Table Comparisons between responders and resistant patients regarding pre-treatment characteristics. Table Laboratory features and disease activity scores prior to intravenous pulse methylprednisolone in patients with different genotypes, models and alleles of the multidrug-resistance 1 gene (MDR) C12s36T and C

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  • Article Type:     Research Article
Keywords: Systemic lupus erythematosus ; multidrug resistance 1 gene ; MDR1 gene ; glucocorticoids

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