Comparison and Analysis of Computational Methods for Identifying N⁶-Methyladenosine Sites in Saccharomyces cerevisiae

Background: N⁶-methyladenosine (m⁶A) plays critical roles in a broad range of biological processes. Knowledge about the precise location of m⁶A site in the transcriptome is vital for deciphering its biological functions. Although experimental techniques have made substantial contributions to identify m⁶A, they are still labor intensive and time consuming. As complement to experimental methods, in the past few years, a series of computational approaches have been proposed to identify m⁶A sites. Methods: In order to facilitate researchers to select appropriate methods for identifying m⁶A sites, it is necessary to conduct a comprehensive review and comparison of existing methods. Results: Since research works on m⁶A in Saccharomyces cerevisiae are relatively clear, in this review, we summarized recent progress of computational prediction of m⁶A sites in S. cerevisiae and assessed the performance of existing computational methods. Finally, future directions of computationally identifying m⁶A sites are presented. Conclusion: Taken together, we anticipate that this review will serve as an important guide for computational analysis of m⁶A modifications.