Skip to content
2000
Volume 8, Issue 13
  • ISSN: 1381-6128
  • E-ISSN: 1873-4286

Abstract

The use of genomics tools to discover new genes, to decipher pathways or to assign a function to a gene is just beginning to have an impact. Genomics approaches have been applied to both antibacterial and antifungal target discovery in order to identify a new generation of antibiotics. This review discusses genomics approaches for antifungal drug discovery, focusing on the areas of gene discovery, target validation, and compound screening. A variety of methods to identify fungal genes of interest are discussed, as well as methods for obtaining full-length sequences of these genes. One approach is well-suited to organisms having few introns (Candida albicans), and another for organisms with many introns (Aspergillus fumigatus). To validate broad spectrum fungal targets, the yeast Saccharomyces cerevisiae was used as a model system to rapidly identify genes essential for growth and viability of the organism. Validated targets were then exploited for high-throughput compound screening.

Loading

Article metrics loading...

/content/journals/cpd/10.2174/1381612023394890
2002-06-01
2025-05-05
Loading full text...

Full text loading...

/content/journals/cpd/10.2174/1381612023394890
Loading

  • Article Type:
    Review Article
Keyword(s): antibacterial; antifungal; bacterial genes; fungal genes; target validation
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test