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Volume 31, Issue 5
  • ISSN: 1381-6128
  • E-ISSN: 1873-4286

Abstract

Background

(PS), the spike from L., is a traditional Chinese medicine that can treat Oral Squamous Cell Carcinoma (OSCC), whereas its molecular mechanisms and effects on the prognosis of patients remain unclear.

Methods

Our study aimed to identify the potential anti-OSCC targets of PS and explore its mechanisms and effects on prognosis through network pharmacology, bioinformatics analysis, molecular docking, and cell assays.

Results

Sixty-two potential targets of 11 active anti-OSCC ingredients of PS were identified, with Quercetin, the core ingredient of PS, exhibiting the most significant number of OSCC-related targets. GO analysis indicated that the primary biological processes involved in OSCC treatment by PS were the cellular response to nitrogen compound, response to xenobiotic stimulus, and cellular response to organonitrogen compound. KEGG analysis revealed that pathways in cancer were the top highly enriched signaling pathway in the treatment of OSCC by PS. DisGeNET analysis is mainly about Lip and Oral Cavity Carcinoma. More importantly, 6 of the 62 targets were markedly related to prognosis. Molecular docking revealed high affinities between the key component and the prognosis-related target proteins. Treatment of OSCC cell line SCC-25 with Quercetin could inhibit malignant biological behaviors, such as cell proliferation, colony formation, invasion, and migration, as well as affect the targets related to prognosis and promote autophagy.

Conclusion

Overall, these results suggest that PS plays a significant role in treating and improving the prognosis of OSCC by directly influencing various processes in OSCC.

© 2025 Bentham Science Publishers
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2023-09-16
2025-04-06

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An Integrated Approach of Network Pharmacology, Bioinformatics, Molecular Docking, and Experimental Verification Uncovers Prunellae Spica as the Potential Medicine of Prognosis Improvement for Oral Squamous Cell Carcinoma
Curr. Pharm. Des. 31, 391 (2025); https://doi.org/10.2174/0113816128328547240827045955
/content/journals/cpd/10.2174/0113816128328547240827045955
/content/journals/cpd/10.2174/0113816128328547240827045955
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  • Article Type:     Research Article
Keyword(s): bioinformatics; moleculr docking; network pharmacology; oral squamous cell carcinoma; Prunellae Spica; Quercetin

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