Current Pharmaceutical Biotechnology - Volume 17, Issue 14, 2016

Cancer, a disease that currently affects approximately 14 million people, is characterized by abnormal cell growth with altered replication capacity, which leads to the development of tumor masses without apoptotic control. Resistance to the drugs used in chemotherapy and their side effects stimulate scientific research seeking new therapies to combat this disease. Molecules from flora and fauna with cytotoxic activity agains Read More

Cell viability is defined as the number of healthy cells in a sample and proliferation of cells is a vital indicator for understanding the mechanisms in action of certain genes, proteins and pathways involved cell survival or death after exposing to toxic agents. Generally, methods used to determine viability are also common for the detection of cell proliferation. Cell cytotoxicity and proliferation assays are generally used for drug scr Read More

Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) is a fluorescence based technique which enables the analysis of molecular interactions in biochemical processes. Principle of TR-FRET is based on time-resolved fluorescence (TRF) measurement and fluorescence resonance energy transfer (FRET) between donor and acceptor molecules. To generate FRET signal, donor and acceptor molecules must show spec Read More

Background: Despite development of novel cancer drugs, invasive ductal breast carcinoma and its metastasis are still highly morbid. Therefore, new therapeutic approaches are being developed and Hsp90 is an important target for drug design. For this purpose, a series of benzodiazepine derivatives were designed and synthesized as novel Hsp90 inhibitor. Methods: Benzodiazepine derivatives anticancer activities were dete Read More

In recent years, many systems biology approaches have been used with various cancers. The materials described here can be used to build bases to discover novel cancer therapy targets in connection with computer-aided drug design (CADD). A deeper understanding of the mechanisms of cancer will provide more choices and correct strategies in the development of multiple target drug therapies, which is quite different fro Read More

Aerosolized medications are commonly used for the treatment of patients with pulmonary diseases in acute care. While there are many drug/device combinations available on the market, new devices have been developed in the past few years. Due to differences in their operation, performance and requirements for proper use, it is important to know how to use these aerosol devices effectively during inhalation the Read More

Background: Phytol (3,7,11,15-tetramethylhexadec-2-en-1-ol; PHY), the alcoholic diterpenoid is particularly interesting due to its diverse activities found in literature. This study evaluated in vitro and in vivo antioxidant capacity of PHY. Methods: We conducted DPPH• (2,2-diphenyl-1-picrylhydrazyl) and ABTS•+ (2,2'-azino-bis(3- ethylbenzthiazoline-6-sulphonic acid)) radical scavenging tests as in vitro, while Saccharom Read More

Background: Plant synthesized silver nanoparticles give rapid control on mosquito larvae of dengue vector, Aedes albopictus. AgNPs synthesized from the plant, Argemone mexicana for the control of larvae and these nanoparticles inhibit the growth of microbes are broad spectrum of nanoparticle activities. Methods: Nanoparticles were subjected to analysis by UV-vis spectrophotometry, Fourier transform infrared spec Read More